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Facile synthesis of polyaniline-polythionine redox hydrogel: Conductive, antifouling and enzyme-linked material for ultrasensitive label-free amperometric immunosensor toward carcinoma antigen-125
Analytica Chimica Acta ( IF 5.7 ) Pub Date : 2018-01-01 , DOI: 10.1016/j.aca.2017.10.017
Lihua Zhao , Zhanfang Ma

Sensitivity enhancement and proteins adsorption are the common challenges faced in protein immunoassays. In this work, an ultrasensitive and protein-resistant label-free amperometric immunosening platform for carcinoma antigen-125 (CA125) based on redox polyaniline-polythionine hydrogel (PANI-PThi gel) was developed. The as-prepared hydrogel, which was facilely synthesized by electropolymerization, exhibited good conductivity and strong hydrophilicity while the sensitivity and specificity of the immunosensor can be enhanced. Furthermore, the as-prepared AuNPs functionalized PANI-PThi gel exhibited strong current signal and H2O2 electrocatalytic ability, which guaranteed a large current variable range. Based on these, the prepared immunosensor revealed a wide linear range from 0.0001 U mL-1 to 1 kU mL-1, a limit of detection of 0.00125 U mL-1 and its sensitivity was at least three-fold higher than previous works. More importantly, the prepared immunosensor exhibited excellent specificity, making it capable of assaying CA125 in human serum.

中文翻译:

聚苯胺-聚硫氨酸氧化还原水凝胶的简便合成:导电、防污和酶联材料,用于针对癌抗原 125 的超灵敏无标记电流免疫传感器

灵敏度增强和蛋白质吸附是蛋白质免疫测定中面临的常见挑战。在这项工作中,开发了一种基于氧化还原聚苯胺-聚硫氨酸水凝胶(PANI-PThi 凝胶)的超灵敏和蛋白质抗性无标记安培免疫检测平台,用于癌抗原-125(CA125)。所制备的水凝胶通过电聚合方法合成,具有良好的导电性和强亲水性,同时可以提高免疫传感器的灵敏度和特异性。此外,所制备的 AuNPs 功能化 PANI-PThi 凝胶表现出强电流信号和 H2O2 电催化能力,保证了较大的电流可变范围。基于这些,制备的免疫传感器显示出从 0.0001 U mL-1 到 1 kU mL-1 的宽线性范围,检测限为 0。00125 U mL-1,其灵敏度至少比以前的作品高出三倍。更重要的是,所制备的免疫传感器表现出优异的特异性,使其能够检测人血清中的 CA125。
更新日期:2018-01-01
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