In this paper, we describe a validated paper-based microfluidic analytical device for the simultaneous quantification of two important biomarkers of renal function in urine. This paper platform provides an inexpensive, simple, and easy to use colorimetric method for the quantification of creatinine (CRN) and uric acid (UA) in urine samples. The microfluidic paper-based analytical device (μPAD) consists of a main channel with three identical arms, each containing a circular testing zone and a circular uptake zone. Creatinine detection is based on the Jaffé reaction, in which CRN reacts with picrate to form an orange-red product. Uric acid quantification is based on the reduction of Fe3+ to Fe2+ by UA, which is detected in a colorimetric reaction using 1,10-phenanthroline. Under optimum conditions, obtained through chemometrics, the concentrations of the analytes showed good linear correlations with the effective intensities, and the method presented satisfactory repeatability. The limits of detection and the linear ranges, respectively, were 15.7 mg L-1 and 50-600 mg L-1 for CRN and 16.5 mg L-1 and 50-500 mg L-1 for UA. There were no statistically significant differences between the results obtained using the μPAD and a chromatographic comparative method (Student's t-test at 95% confidence level).

中文翻译：

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使用经过验证的纸质微流体分析设备同时测定尿液中的肾功能生物标志物

在本文中，我们描述了一种经过验证的纸质微流体分析装置，用于同时量化尿液中肾功能的两个重要生物标志物。该纸平台提供了一种廉价、简单且易于使用的比色方法，用于定量尿液样本中的肌酐 (CRN) 和尿酸 (UA)。微流体纸基分析装置 (μPAD) 由具有三个相同臂的主通道组成，每个臂包含一个圆形测试区和一个圆形摄取区。肌酐检测基于 Jaffé 反应，其中 CRN 与苦味酸盐反应形成橙红色产物。尿酸定量基于 UA 将 Fe3+ 还原为 Fe2+，UA 在比色反应中使用 1,10-菲咯啉进行检测。在通过化学计量学获得的最佳条件下，分析物的浓度与有效强度显示出良好的线性相关性，并且该方法具有令人满意的重复性。CRN 的检测限和线性范围分别为 15.7 mg L-1 和 50-600 mg L-1，UA 分别为 16.5 mg L-1 和 50-500 mg L-1。使用 μPAD 和色谱比较方法（95% 置信水平的学生 t 检验）获得的结果之间没有统计学上的显着差异。