The new sorbent for solid phase extraction (SPE), based on silica gel modified with a copper (II), was obtained and its application for phospholipids removal from the human plasma was tested. SPE column conditioning requirements, the volume of the plasma, the composition of the elution solvent were all established. The efficacy of the removal of phospholipids was compared for different methods such as standard protein precipitation or HybridSPE Phospholipid Ultra and HybridSPE-PPT. The sample clean-up was verified by mass spectrometry (MS) and by monitoring of chromatograms in the region between 190 nm and 400 nm. The Fourier Transform Infrared Spectroscopy FT-IR and confocal Raman microscopy were used to evaluating the silica gel modifications and to show the structure of lipids confined in the silica pores.

获得了一种新的固相萃取吸附剂（SPE），该吸附剂基于铜（II）修饰的硅胶，并测试了其在人体血浆中去除磷脂的应用。确定了SPE柱的条件，血浆体积，洗脱溶剂的组成。比较了不同方法（例如标准蛋白沉淀或HybridSPE磷脂Ultra和HybridSPE-PPT）去除磷脂的功效。通过质谱（MS）和监测190 nm至400 nm之间区域的色谱图来验证样品净化效果。傅立叶变换红外光谱FT-IR和共聚焦拉曼显微镜用于评估硅胶的改性，并显示限制在硅胶孔中的脂质的结构。