In this study, a QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) method, optimized by a 2³ full factorial design, was developed for the determination of 72 pesticides in plant parts of carrot, corn, melon, rice, soy, silage, tobacco, cassava, lettuce and wheat by ultra-high-performance liquid chromatographic tandem mass spectrometry (UHPLC–MS/MS). Considering the complexity of these matrices and the need of use calibration in matrix, a new calibration approach based on single level standard addition in the sample (SLSAS) was proposed in this work and compared with the matrix-matched calibration (MMC), the procedural standard calibration (PSC) and the diluted standard addition calibration (DSAC). All approaches presented satisfactory validation parameters with recoveries from 70 to 120% and relative standard deviations ≤ 20%. SLSAS was the most practical from the evaluated approaches and proved to be an effective way of calibration. Method limit of detection were between 4.8 and 48 μg kg⁻¹ and limit of quantification were from 16 to 160 μg kg⁻¹. Method application to different kinds of plants found residues of 20 pesticides that were quantified with z-scores values ≤ 2 in comparison with other calibration approaches. The proposed QuEChERS method combined with UHPLC–MS/MS analysis and using an easy and effective calibration procedure presented satisfactory results for pesticide residues determination in different crop plants and is a good alternative for routine analysis.

在这项研究中，QuEChERS（快速，简便，便宜，有效，坚固和安全）方法通过2 ³进行了优化采用超高效液相色谱串联质谱法（UHPLC–MS），开发了全因子设计，用于测定胡萝卜，玉米，瓜，大米，大豆，青贮饲料，烟草，木薯，生菜和小麦的植物部位中的72种农药/多发性硬化症）。考虑到这些矩阵的复杂性以及在矩阵中使用校准的需求，在这项工作中，提出了一种基于样品中单水平标准品添加的新校准方法（SLSAS），并将其与矩阵匹配校准（MMC）进行了比较。标准校准（PSC）和稀释的标准添加校准（DSAC）。所有方法均提供令人满意的验证参数，回收率从70％到120％，相对标准偏差≤20％。从评估的方法来看，SLSAS是最实用的方法，并且被证明是一种有效的校准方法。^-1和定量限为16至160μgkg ^-1。在不同种类植物上的方法应用发现了与其他校准方法相比z得分≤2的20种农药残留。拟议的QuEChERS方法与UHPLC-MS / MS分析相结合，并使用简单有效的校准程序，为不同农作物中农药残留的测定提供了令人满意的结果，是常规分析的良好替代方法。