Correlations in the elemental and metabolic profiles of the lichen Dirinaria picta after road traffic exposure,Metallomics

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Correlations in the elemental and metabolic profiles of the lichen Dirinaria picta after road traffic exposure
Metallomics ( IF 2.9 ) Pub Date : 2017-10-26 00:00:00 , DOI: 10.1039/c7mt00207f
Xulei Huang _{1,

2,

3} , Lei Wang _{1,

2,

3} , Anna Karen Carrasco Laserna _{1,

2,

3} , Sam Fong Yau Li _{1,

2,

3,

4,

5}

Affiliation

Lichens can be used as cost-effective biomonitors of elements from road traffic in the urban environment. However, the nature of comprehensive interactions between hazardous heavy metals from road traffic and lichen metabolites remains unclear. In this study, elemental and metabolic profiles of the lichen Dirinaria picta after exposure to road traffic for 3 months in 9 sites of Singapore city were investigated. Concentrations of 34 elements reveal strong correlations among each other and a general increase with traffic exposure level. A variety of metabolites, i.e. sugar alcohols, sugars, amino acids, low-molecular mass organic acids, secondary metabolites and nucleosides, are identified through a multi-platform approach combining liquid chromatography quadrupole time-of-flight mass spectrometry (LC-QTOF/MS), gas chromatography quadrupole mass spectrometry (GC-Q/MS) and ¹H nuclear magnetic resonance (¹H NMR). The total amount of the heavy metals Fe, Zn, Cu, Cd, Pb and Co can be significantly correlated with 21 metabolites positively and 9 metabolites negatively. These correlations reveal a heavy metal detoxification mechanism through ROS scavenging, osmoregulation and chelation with N atoms and sulfhydryl groups, as well as depletion of arabitol and inhibited synthesis of certain secondary metabolites such as quinones and steroids due to heavy metal stress. This study provides new insights into the metabolic toxicity and detoxification mechanisms in lichens under heavy metal exposure.

中文翻译：

道路交通暴露后地衣Dirinaria picta元素和代谢谱的相关性

地衣可用作城市环境中道路交通中元素的具有成本效益的生物监测器。然而，道路交通中的有害重金属与地衣代谢物之间全面相互作用的性质仍不清楚。在这项研究中，调查了新加坡9个地点的道路交通3个月后，地衣Dirinaria picta的元素和代谢谱。34种元素的浓度相互之间显示出很强的相关性，并且随着交通暴露水平的增加而总体增加。多种代谢物，即糖醇，糖，氨基酸，低分子量有机酸，次生代谢产物和核苷通过多平台方法结合液相色谱四极杆飞行时间质谱（LC-QTOF / MS），气相色谱四极杆进行鉴定质谱（GC-Q / MS）和¹ H核磁共振（¹1 H NMR）。重金属Fe，Zn，Cu，Cd，Pb和Co的总量与21种代谢物呈正相关，而9种代谢物呈负相关。这些相关性揭示了通过ROS清除，与N原子和巯基的渗透作用和螯合作用，以及阿拉伯糖醇的消耗和由于重金属胁迫而抑制某些次生代谢产物如醌和类固醇合成的重金属解毒机理。这项研究为重金属暴露下的地衣的代谢毒性和排毒机理提供了新的见解。

更新日期：2017-10-26

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