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Portable GMR Handheld Platform for the Detection of Influenza A Virus
ACS Sensors ( IF 8.2 ) Pub Date : 2017-10-25 00:00:00 , DOI: 10.1021/acssensors.7b00432
Kai Wu 1 , Todd Klein 1 , Venkatramana D. Krishna 2 , Diqing Su 1, 3 , Andres M. Perez 2 , Jian-Ping Wang 1
Affiliation  

Influenza A virus (IAV) is a common respiratory pathogen infecting many hosts including humans, pigs (swine influenza virus or SIV), and birds (avian influenza virus or AIV). Monitoring swine and avian influenza viruses in the wild, farms, and live poultry markets is of great significance for human and veterinary public health. A portable, sensitive, and quantitative immunoassay device will be of high demand especially in the rural and resource-limited areas. We report herein our Z-Lab point-of-care (POC) device for sensitive and specific detection of swine influenza viruses with minimum sample handling and laboratory skill requirements. In the present study, a portable and quantitative immunoassay platform based on giant magnetoresistive (GMR) technology is used for the detection of IAV nucleoprotein (NP) and purified H3N2v. Z-Lab displays quantitative results in less than 10 min with sensitivities down to 15 ng/mL and 125 TCID50/mL for IAV nucleoprotein and purified H3N2v, respectively. This platform allows lab-testing to be performed outdoors and opens up the applications of immunoassays in nonclinical settings.

中文翻译:

用于检测甲型流感病毒的便携式GMR手持平台

甲型流感病毒(IAV)是一种常见的呼吸道病原体,可感染许多宿主,包括人,猪(猪流感病毒或SIV)和鸟类(禽流感病毒或AIV)。监视野生,农场和活禽市场中的猪和禽流感病毒对人类和兽医公共卫生具有重要意义。便携式,灵敏和定量的免疫测定设备将是特别需要的,特别是在农村和资源有限的地区。我们在这里报告我们的Z-Lab即时医疗(POC)设备,以最少的样品处理和实验室技能要求对猪流感病毒进行灵敏和特异的检测。在本研究中,基于巨磁阻(GMR)技术的便携式定量免疫测定平台用于检测IAV核蛋白(NP)和纯化的H3N2v。IAV核蛋白和纯化的H3N2v分别为50 / mL。该平台允许在户外进行实验室测试,并开辟了免疫测定在非临床环境中的应用。
更新日期:2017-10-25
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