The endothelial lining of blood vessels is severely affected in type II diabetes. Yet, there is still a paucity on the use of diabetic endothelial cells for study and assessment of implantable devices targeting vascular disease. This critically impairs our ability to determine appropriate topographical cues to be included in implantable devices that can be used to maintain or improve endothelial cell function in vivo. Here, the functional responses of healthy and diabetic human coronary arterial endothelial cells were studied and observed to differ depending on topography. Gratings (2 μm) maintained normal endothelial functions such as adhesiveness, angiogenic capacity and cell-cell junction formation, and reduced immunogenicity of healthy cells. However, a significant and consistent effect was not observed in diabetic cells. Instead, diabetic endothelial cells cultured on the perpendicularly aligned multi-scale hierarchical gratings (250 nm gratings on 2 μm gratings) drastically reduced the uptake of oxidized low-density lipoprotein, decreased immune activation, and accelerated cell migration. Concave microlens (1.8 μm diameter) topography were additionally observed to overwhelmingly deteriorate diabetic endothelial cell function. The results of this study support a new paradigm and approach in the design and testing of implantable devices and biomedical interventions for diabetic patients.

中文翻译：

---

健康和糖尿病内皮细胞在地形提示上的功能差异

血管内皮层在II型糖尿病中受到严重影响。然而，仍然缺乏使用糖尿病内皮细胞来研究和评估靶向血管疾病的可植入装置。这严重损害了我们确定可植入设备中包含的适当地形线索的能力，这些设备可用于在体内维持或改善内皮细胞的功能。在这里，研究了健康的和糖尿病的人冠状动脉内皮细胞的功能反应，并根据地形不同而有所不同。光栅（2μm）维持正常的内皮功能，例如粘附性，血管生成能力和细胞间连接形成，并降低了健康细胞的免疫原性。然而，在糖尿病细胞中未观察到显着且一致的作用。取而代之的是，在垂直排列的多尺度分层光栅（2μm光栅上为250 nm光栅）上培养的糖尿病内皮细胞会大大减少氧化型低密度脂蛋白的摄取，降低免疫激活，并加速细胞迁移。此外，还观察到凹形微透镜（直径为1.8μm）的形貌使糖尿病内皮细胞功能严重恶化。