Tumor Necrosis Factor Alpha (TNFα) is an important marker of inflammatory processes in human body. In the current healthcare, determination of TNFα blood or plasma level is done by Enzyme Linked Immuno-Sorbent Assay (ELISA) as a primary choice method. Piezoelectric immunosensors are analytical platform recording affinity interactions on their surface. It is inferred that the immunosensors would be a functional alternative to the ELISA. In this study, antibody against TNFα was immobilized on Quartz Crystal Microbalance (QCM) sensor and the same was made on magnetic particles. Human TNFα was measured in a way of interaction with QCM surface and then the particles were applied. The assay exerted sufficient limit of detection equal to 1.62 pg/ml and it also fully correlated with standard ELISA tests. No interference by interleukin 6 or albumin was observed. Long term stability of the immunosensors lasting for at least three months was found. The immunosensors appears to be readily for practical performance and it would be an alternative to the standard ELISA especially when diagnoses made in field, homecare conditions or conditions of small hospitals as an emergency test.

肿瘤坏死因子α（TNFα）是人体炎症过程的重要标志。在当前的医疗保健中，通过酶联免疫吸附测定（ELISA）作为主要选择方法来确定TNFα血液或血浆水平。压电免疫传感器是分析平台，可记录其表面上的亲和力相互作用。可以推断，免疫传感器将是ELISA的功能替代品。在这项研究中，针对TNFα的抗体被固定在石英晶体微天平（QCM）传感器上，并且在磁性颗粒上也被制成。以与QCM表面相互作用的方式测量人TNFα，然后施加颗粒。该测定法的检测下限足以达到1.62 pg / ml，并且还与标准ELISA检测完全相关。没有观察到白介素6或白蛋白的干扰。发现免疫传感器可以持续至少三个月的长期稳定性。免疫传感器似乎很容易实现实用性能，它将替代标准ELISA，尤其是当在野外，家庭护理条件或小型医院的条件下进行诊断作为紧急测试时。