Targeting ligand-functionalized photothermal scaffolds for cancer cell capture and in situ ablation,Biomaterials Science

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Targeting ligand-functionalized photothermal scaffolds for cancer cell capture and in situ ablation
Biomaterials Science ( IF 5.8 ) Pub Date : 2017-09-21 00:00:00 , DOI: 10.1039/c7bm00639j
Jing Zhang _{1,

2,

3,

4,

5} , Jingchao Li _{1,

2,

3,

4,

5} , Xiuhui Wang _{1,

2,

3,

4,

5} , Naoki Kawazoe _{1,

2,

3,

4} , Guoping Chen _{1,

2,

3,

4,

5}

Affiliation

Scaffolds have been explored as a useful carrier to deliver efficient photothermal conversion agents for localized photothermal therapy applications because they can confine the agents in designated regions. However, cell capture by porous structures cannot discriminate between cancer cells and normal cells. In this study, folic acid (FA) as a typical targeting ligand for cancer cells was introduced into the porous photothermal scaffolds. Poly-L-lysine was mixed with gelatin to increase the amino side groups for FA incorporation. By changing the poly-L-lysine amount, the number of free amino groups in the scaffolds could be controlled. After the reaction with activated FA, composite scaffolds incorporated with different amounts of FA were prepared. Cell culture results showed that the FA-functionalized photothermal scaffolds could efficiently capture folate-positive cervical cancer cells compared with gelatin scaffolds. The capture efficiency increased with the increase in the amount of FA in the scaffolds. Furthermore, cancer cells in the FA-functionalized photothermal scaffolds can be efficiently killed during near-infrared laser irradiation. The results indicated that the FA-functionalized photothermal scaffolds had good cancer cell capture ability and excellent cancer cell ablation efficacy, which may provide useful information for the design of multifunctional scaffolds with cancer cell capture capacity and photothermal ablation ability.

中文翻译：

靶向配体功能化的光热支架，用于癌细胞捕获和原位消融

支架被探索为递送有效的光热转化剂以用于局部光热疗法应用的有用载体，因为它们可以将试剂限制在指定区域中。但是，多孔结构的细胞捕获不能区分癌细胞和正常细胞。在这项研究中，叶酸（FA）作为癌细胞的典型靶向配体被引入到多孔光热支架中。将聚-L-赖氨酸与明胶混合以增加用于FA掺入的氨基侧基。通过改变poly- L-赖氨酸的量，可以控制支架中游离氨基的数目。与活化的FA反应后，制备掺有不同量的FA的复合支架。细胞培养结果表明，与明胶支架相比，FA功能化的光热支架可以有效捕获叶酸阳性子宫颈癌细胞。捕获效率随支架中FA含量的增加而增加。此外，可以在近红外激光辐照期间有效地杀死FA-功能化的光热支架中的癌细胞。结果表明，FA官能化的光热支架具有良好的癌细胞捕获能力和优异的癌细胞消融功效，

更新日期：2017-10-24

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