For the first time, a sensitive conductive nanobiocomposite sensor consisting of Au–In₂O₃ nanocomposite and chitosan (CS) was successfully prepared and used for the modification of acetylene black paste electrode (Au-­In₂O₃­-CS/ABPE). The phase structures, composition and morphology of Au–In₂O₃ nanocomposite were characterized by X-ray diffraction (XRD), energy–dispersed X-ray (EDX) and transmission electron microscopy (TEM). Electrochemical activities and surface analysis of the biosensor electrode Au­-In₂O₃­-CS/ABPE were investigated using scanning electron microscopy (SEM), cyclic voltammetry and square wave voltammetry. The modified electrode showed an excellent electrochemical activity toward the electro–oxidation of the antimycotic ciclopirox olamine (CPX) leading to a significant improvement in sensitivity as compared to the bare ABPE. The proposed biosensor demonstrated linearity in the range 0.199 – 16.22 µmol L⁻¹, with high sensitivity (64.57 μA µmol L⁻¹ cm⁻²) and detection limit of 6.64 × 10⁻⁹ mol L⁻¹ CPX. The analytical performance of this biosensor was evaluated for detection of CPX in pharmaceutical formulations with good accuracy and precision. This proposed method was validated by UPLC and the results are in agreement at the 95% confidence level.

首次成功地制备了由Au–In ₂ O ₃纳米复合材料和壳聚糖（CS）组成的灵敏导电纳米生物复合传感器，并将其用于乙炔黑糊电极（Au-In ₂ O ₃ -CS / ABPE）的改性。通过X射线衍射（XRD），能量分散X射线（EDX）和透射电子显微镜（TEM）对Au–In ₂ O ₃纳米复合材料的相结构，组成和形貌进行了表征。生物传感器电极Au-In ₂ O _3的电化学活性和表面分析-CS / ABPE使用扫描电子显微镜（SEM），循环伏安法和方波伏安法进行了研究。与裸露的ABPE相比，修饰后的电极对抗真菌的环吡酮醇胺（CPX）的电氧化表现出优异的电化学活性，从而导致灵敏度显着提高。拟议的生物传感器显示出0.199 – 16.22 µmol L ^-1的线性度，灵敏度高（64.57μAµmol L ^-1  cm ^-2），检测极限为6.64×10 ^-9  mol L ^-1CPX。评估了该生物传感器的分析性能，可用于以良好的准确性和精密度检测药物制剂中的CPX。UPLC验证了该方法的有效性，结果在95％的置信度水平上是一致的。