Cavitation hybrid rotation, which was and is still looked upon as an unavoidable nuisance in the flow systems, for extraction processing intensification of active chemical compounds from natural products. In this study, a homogenization-assisted cavitation hybrid rotation extraction method was applied to extract dihydroquercetin (DHQ) from larch (Larix gmelinii) wood root. The extraction parameters were optimized in single factor experiments with the DHQ extraction yields as the response values. The optimum conditions were as follows: number of extractions, three; ethanol volume fraction for the extraction, 60%; liquid–solid ratio for homogenization, 10 mL/g; homogenization time, 8 min; liquid–solid ratio for cavitation extraction, 9 mL/g, and cavitation extraction time, 35 min. Under these conditions, the DHQ content in extract was 4.50 ± 0.02 mg/g, and the extraction efficiency was higher than those of traditional techniques. Cavitation can be effectively used to improve the extraction rate by increasing the mass transfer rates and possible rupture of cell wall due to formation of microcavities leading to higher product yields with reduced processing time and solvent consumption. After the extraction process, macroporous resin column chromatography was used to concentrate and purify the DHQ. Three resins were selected from fifteen macroporous resins for further investigation of their performance. Among these resins, AB-8 resin exhibited relatively better adsorption capacities and desorption ratios for DHQ. The ethanol volume fraction of the solutions for sample loading and desorption, and flow rates for loading and desorption were optimized for the macroporous resin column chromatography.

空化混合旋转曾经是并且仍然被认为是流动系统中不可避免的麻烦，用于从天然产物中提取活性化合物。本研究采用匀浆辅助空化混合旋转萃取法从落叶松（落叶松）中提取二氢槲皮素（DHQ）。）木根。在单因素实验中以DHQ提取得率作为响应值对提取参数进行了优化。最佳条件如下：提取次数为3次；提取次数为3次。提取的乙醇体积分数为60％；均质的液固比为10 mL / g；均质时间8分钟；空化萃取的液固比为9 mL / g，空化萃取时间为35分钟。在此条件下，提取物中的DHQ含量为4.50±0.02 mg / g，提取效率高于传统技术。通过增加传质速率以及由于微腔的形成而导致的细胞壁破裂，可以有效地利用空化来提高提取率，从而导致更高的产品收率，同时减少了处理时间和溶剂消耗。提取过程后，使用大孔树脂柱色谱法浓缩和纯化DHQ。从15种大孔树脂中选择了3种树脂，以进一步研究其性能。在这些树脂中，AB-8树脂对DHQ表现出相对较好的吸附容量和解吸率。对于大孔树脂柱色谱，优化了用于样品上样和解吸的溶液的乙醇体积分数，以及上样和解吸的流速。