Astragali Radix, the root of Astragalus membranaceus Bunge, is one of the most frequently used crude drugs in Asian medicine. We developed a quantification method for 6 components (calycosin, formononetin, astragaloside I–IV) of Astragali Radix and Hwanggi-gyeji-omul-tang (HGOT) using reverse-phase high-performance liquid chromatography coupled with integrated pulsed amperometric detection (RP-HPLC-IPAD). The plants were extracted in 80% ethanol for 2 h. All target components were detected with good sensitivity using sodium hydroxide (as a post-column eluent). The limit of detection (S/N = 3) and limit of quantification (S/N = 10) of the target components ranged from 0.10–1.00 ng and from 0.30–3.00 ng, respectively. The coefficients of linear regression ranged from 0.9993–1.0000, all interday and intraday precision values were <3.64%, and the average recovery ranged from 99.00–102.97% for Astragali Radix and 97.73–102.57% for HGOT. This method exhibited good selectivity, sensitivity, and reproducibility and can be used directly without any pretreatment steps. Our method will therefore be useful as a quality control measure for Astragali Radix.

黄芪的根，黄芪邦吉，是亚洲医学中最常用的药材之一。我们使用反相高效液相色谱结合集成的脉冲安培检测法（RP- HPLC-IPAD）。将植物在80％乙醇中萃取2小时。使用氢氧化钠（作为柱后洗脱液）以良好的灵敏度检测到所有目标组分。检出限（S / N  =  3）和定量限（S / N  = 10）的目标成分分别在0.10–1.00 ng和0.30–3.00 ng之间。线性回归系数范围为0.9993-1.0000，所有日间和日内精度值均<3.64％，Astragali Radix和HGOT的平均回收率范围分别为99.00-102.97％和97.73-102.57％。该方法具有良好的选择性，灵敏度和重现性，无需任何预处理步骤即可直接使用。因此，我们的方法将用作黄芪的质量控制手段。