For many sample matrices, matrix effects are a troublesome phenomenon using the electrospray ionization source. The increasing use of supercritical fluid chromatography with CO2 in combination with the electrospray ionization source for MS detection is therefore raising questions: is the matrix effect behaving differently using SFC in comparison with reversed phase LC? This was investigated using urine, plasma, influent- and effluent-wastewater as sample matrices. The matrix effect was evaluated using the post-extraction addition method and through post-column infusions. Matrix effect profiles generated from the post-column infusions in combination with time of flight-MS detection provided the most valuable information for the study. The combination of both qualitative and semi-quantitative information with the ability to use HRMS-data for identifying interfering compounds from the same experiment was very useful, and has to the authors' knowledge not been used this way before. The results showed that both LC and SFC are affected by matrix effects, however differently depending on sample matrix. Generally, both suppressions and enhancements were seen, with a higher amount of enhancements for LC, where 65% of all compounds and all sample matrices were enhanced, compared to only 7% for SFC. Several interferences were tentatively identified, with phospholipids, creatinine, and metal ion clusters as examples of important interferences, with different impact depending on chromatographic technique. SFC needs a different strategy for limiting matrix interferences, owing to its almost reverse retention order compared to RPLC.

中文翻译：

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超临界流体色谱与ESI/MS联用反相液相色谱的基质效应差异

对于许多样品基质，使用电喷雾电离源时，基质效应是一个麻烦的现象。因此，越来越多地使用 CO2 超临界流体色谱与电喷雾电离源相结合进行 MS 检测提出了问题：与反相 LC 相比，使用 SFC 的基质效应表现是否不同？使用尿液、血浆、流入和流出废水作为样品基质对此进行了研究。使用萃取后添加方法和柱后灌注来评估基质效应。柱后注入产生的基质效应曲线与飞行时间 MS 检测相结合，为研究提供了最有价值的信息。将定性和半定量信息与使用 HRMS 数据从同一实验中识别干扰化合物的能力相结合非常有用，据作者所知，以前没有以这种方式使用过。结果表明 LC 和 SFC 都受基质效应的影响，但因样品基质而异。一般来说，抑制和增强都可以看到，LC 的增强量更高，其中 65% 的所有化合物和所有样品基质得到增强，而 SFC 仅为 7%。初步确定了几种干扰，以磷脂、肌酐和金属离子簇作为重要干扰的例子，根据色谱技术具有不同的影响。SFC 需要不同的策略来限制基质干扰，