Selenium is an essential element of which the deficiency is often compensated for by supplementation with products based on yeast grown on Se-rich media. Advances in HPLC with element- and molecule-specific detection by mass spectrometry have resulted in evidence of the presence of hundreds of selenocompounds in Se-rich yeast, with different properties and in a large concentration range. This spurs interest in extending –omics-type approaches to selenium speciation analysis to other samples with the ultimate goal to confer biological significance to the obtained data. This review critically evaluates the recent advances in selenium speciation analysis, especially in terms of sensitivity and specificity of ICP-MS and electrospray-MS, and, in terms of resolution, mass accuracy and multistage fragmentation by electrospray-MS. A focus is made on the evolution of the perception of the competitiveness and complementarity of ICP-MS and electrospray-MS detection in HPLC for qualitative and quantitative Se speciation analysis.

硒是必不可少的元素，通常可通过补充基于富含硒的培养基上生长的酵母的产品来弥补硒的不足。通过质谱法进行元素和分子特异性检测的HPLC进展表明，富硒酵母中存在数百种硒化合物，这些化合物具有不同的特性，且浓度范围较大。这激发了将组学类型的硒形态分析方法扩展到其他样品的兴趣，其最终目的是为获得的数据赋予生物学意义。这篇评论评论性地评估了硒形态分析的最新进展，特别是在ICP-MS和电喷雾MS的灵敏度和特异性方面，以及在分辨率，质量准确性和电喷雾MS的多级碎裂方面。