Chelidonium majus L. (greater celandine) has been used as an herbal medicine for several centuries. This study investigated an efficient method to purify chelidonine from the extract of C. majus L. using macroporous adsorption resins and evaluated the antifungal activity of chelidonine against Botryosphaeria dothidea as a model strain. Static adsorption and desorption tests revealed that D101 was the optimal resin for chelidonine purification. Pseudo-second-order kinetics model and Freundlich equation model were the most suitable for evaluating the endothermic and spontaneous adsorption processes of chelidonine on D101. Dynamic adsorption and desorption tests on D101 columns showed that the concentration of chelidonine increased 14.16-fold, from 2.67% to 37.81%, with the recovery yield of 80.77%. The antifungal activity of enriched chelidonine products was studied with B. dothidea. The results showed that the EC₅₀ of crude extracts, enriched chelidonine products, and chelidonine standard against B. dothidea were 3.24 mg/mL, 0.43 mg/mL, and 0.77 mg/mL, respectively. The result of antifungal activity test showed that chelidonine had the potential to be a useful antifungal agent. Moreover, the enrichment method of chelidonine was highly efficient, low cost, and harmless to the environment for industrial applications.

白屈菜（Chelidonium majus L。）（大白屈菜）已被用作草药已有好几个世纪了。本实验研究一种有效的方法来纯化白屈菜碱从提取物C.草使用大孔吸附树脂L.和评价白屈菜碱的针对抗真菌活性杨树dothidea侵染作为模型应变。静态吸附和解吸试验表明，D101是纯化氯胺酮的最佳树脂。伪二级动力学模型和Freundlich方程模型最适合于评价氯丹宁在D101上的吸热和自发吸附过程。在D101色谱柱上进行动态吸附和解吸试验，结果显示，氯胺酮的浓度增加了14.16倍，从2.67％增至37.81％，回收率达80.7％。用多孢杆菌（B. dothidea）研究了富含氯代宁产品的抗真菌活性。结果表明，粗提物，富集的鸟油碱产品和鸟油碱标准品对EC的EC ₅₀达到了B. dothidea。分别为3.24 mg / mL，0.43 mg / mL和0.77 mg / mL。抗真菌活性测试的结果表明，螯胺碱具有成为有用的抗真菌剂的潜力。此外，白屈菜碱的富集方法高效，低成本且对工业应用环境无害。