The aim of the present research is to propose a new method based on localized surface plasmon resonance (LSPR) for fast dengue virus detection. A pool with four dengue serotypes (DENV-1, -2, -3, -4) was detected through antigen-antibody binding using gold nanoparticles (AuNPs) as signaling antibody carriers. Such result was confirmed through surface plasmon resonance (SPR), transmission electron microcopy (TEM), and dynamic light scattering (DLS) techniques. The limit of detection was calculated for TCID₅₀ 10⁷ demonstrating a linear correlation between viral concentration and number of cells with an r² value of > 0.993. The assay presented good sensibility and reproducibility of results and the negative controls were not mistakenly detected. This design requires no pretreatment or high trained person. In the future, it can be used in commercial antibody detection kits.

本研究的目的是提出一种基于局部表面等离振子共振（LSPR）的快速登革热病毒检测方法。使用金纳米颗粒（AuNPs）作为信号抗体载体通过抗原-抗体结合检测出具有四种登革热血清型（DENV-1，-2，-3，-4）的库。通过表面等离振子共振（SPR），透射电子显微镜（TEM）和动态光散射（DLS）技术证实了这一结果。计算出TCID ₅₀ 10 ⁷的检出限，证明病毒浓度与r ²的细胞数之间存在线性关系值> 0.993。该测定法显示了良好的灵敏度和结果再现性，并且没有错误地检测出阴性对照。这种设计不需要预处理或训练有素的人。将来，它可用于商业抗体检测试剂盒。