Bowman-Birk inhibitor (BBI) is a soybean-derived protease inhibitor that has anti-inflammation and anti-HIV effect. Here, we further investigated the anti-HIV action of BBI in macrophages, focusing on its effect on viral entry. We found that BBI could significantly block HIV entry into macrophages. Investigation of the mechanism(s) of the BBI action on HIV inhibition showed that BBI down-regulated the expression of CD4 receptor (as much as 80%) and induced the production of the CC chemokines (up to 60 folds at protein level) in macrophages. This inhibitory effect of BBI on HIV entry could be blocked by the neutralization antibodies to CC chemokines. These findings indicate that BBI may have therapeutic potential as a viral entry inhibitor for the prevention and treatment of HIV infection.

中文翻译：

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大豆衍生的Bowman-Birk抑制剂（BBI）可以阻止HIV进入巨噬细胞。

Bowman-Birk抑制剂（BBI）是一种大豆衍生的蛋白酶抑制剂，具有抗发炎和抗HIV的作用。在这里，我们进一步研究了BBI在巨噬细胞中的抗HIV作用，重点是其对病毒进入的影响。我们发现BBI可以显着阻止HIV进入巨噬细胞。对BBI抑制HIV的作用机制的研究表明，BBI下调了CD4受体的表达（高达80％）并诱导了CC趋化因子的产生（在蛋白水平上高达60倍）。巨噬细胞。BBI对HIV进入的这种抑制作用可以被CC趋化因子的中和抗体所阻断。这些发现表明，BBI作为病毒进入抑制剂可以预防和治疗HIV感染具有治疗潜力。