Biological tissues are recurrently exposed to several dynamic mechanical forces that influence cell behavior. On this work, the focus is on the shear stress forces induced by fluid flow. The study of flow‐induced effects on cells leads to important advances in cardiovascular, cancer, stem cell, and bone biology understanding. These studies are performed using cell culture flow (CCF) systems, mainly parallel plate flow chambers (PPFC), and microfluidic systems. Here, it is proposed an original CCF system based on the open fluidics concept. The system is developed using a planar superhydrophobic platform with hydrophilic paths. The paths work as channels to drive cell culture medium flows without using walls for liquid confinement. The liquid streams are controlled just based on the wettability contrast. To validate the concept, the effect of the shear stress stimulus in the osteogenic differentiation of C2C12 myoblast cells is studied. Combining bone morphogenic protein (specifically BMP‐2) stimulation with this mechanical stimulus, a synergistic effect is found on osteoblast differentiation. This effect is confirmed by the enhancement of alkaline phosphatase activity, a well‐known early marker of osteogenic differentiation. The suggested CCF system combines characteristics and advantages of both the PPFC and microfluidic systems.

中文翻译：

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开放式流体技术：在基于可湿性对比的芯片上开发的细胞培养流程系统

生物组织经常暴露于几种影响细胞行为的动态机械力。在这项工作中，重点是由流体流动引起的切应力。流动诱导作用对细胞的研究导致了心血管，癌症，干细胞和骨骼生物学方面的重要进展。这些研究是使用细胞培养液（CCF）系统（主要是平行板流动室（PPFC））和微流体系统进行的。在此，提出了一种基于开放式流体学概念的原始CCF系统。该系统是使用具有亲水路径的平面超疏水平台开发的。这些路径用作驱动细胞培养基流动的通道，而无需使用壁进行液体限制。仅基于润湿性对比来控制液流。为了验证这个概念，研究了剪切应力刺激对C2C12成肌细胞分化的影响。将骨形态发生蛋白（特别是BMP-2）刺激与这种机械刺激相结合，在成骨细胞分化中发现了协同作用。碱性磷酸酶活性的增强证实了这一作用，碱性磷酸酶活性是成骨分化的著名早期标记。建议的CCF系统结合了PPFC和微流体系统的特性和优势。成骨分化的著名早期标记。建议的CCF系统结合了PPFC和微流体系统的特性和优势。成骨分化的著名早期标记。建议的CCF系统结合了PPFC和微流体系统的特性和优势。