Lack of prominent developmental defects arising from loss of many individual miRNAs is consistent with the observations of collaborative networks between miRNAs and roles for miRNAs in regulating stress responses. However, these characteristics may only partially explain the seemingly nonessential nature of many miRNAs. Non-miRNA gene expression regulatory mechanisms also collaborate with miRNA-induced silencing complex (miRISC) to support robust gene expression dynamics. Genetic enhancer screens have revealed roles of miRNAs and other gene repressive mechanisms in development or other cellular processes that were masked by genetic redundancy. Besides discussing the breadth of the non-miRNA genes, we use LIN-28 as an example to illustrate how distinct regulatory systems, including miRNAs and multiple protein stability mechanisms, work at different levels to target expression of a given gene and provide tissue-specific and stage-specific regulation of gene expression.

由许多单个miRNA的缺失引起的显着发育缺陷的缺乏与miRNA之间的协作网络以及miRNA在调节应激反应中的作用的观察一致。但是，这些特征可能仅部分解释了许多miRNA的看似无关紧要的性质。非miRNA基因表达调控机制还可以与miRNA诱导的沉默复合体（miRISC）合作，以支持强大的基因表达动力学。遗传增强子筛选揭示了miRNA和其他基因抑制机制在发育或其他被遗传冗余掩盖的细胞过程中的作用。除了讨论非miRNA基因的广度之外，我们以LIN-28为例，说明了不同的调控系统（包括miRNA和多种蛋白质稳定性机制）如何，