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Creating Oxidase–Peroxidase Fusion Enzymes as a Toolbox for Cascade Reactions
ChemBioChem ( IF 2.6 ) Pub Date : 2017-10-11 06:25:20 , DOI: 10.1002/cbic.201700478
Dana I. Colpa 1 , Nikola Lončar 2 , Mareike Schmidt 1 , Marco W. Fraaije 1
Affiliation  

Enzyme matchmaking: A set of unnatural bifunctional oxidase–peroxidases fusion enzymes have been created through protein engineering. The artificial enzymes retain the capability to bind the two required cofactors and are catalytically active as oxidases and peroxidases. These fusion enzymes can be used for biosensing purposes or for dioxygen-driven, one-pot, two-step cascade oxidation reactions.

中文翻译:

创建氧化酶-过氧化物酶融合酶作为级联反应的工具箱

酶配对:通过蛋白质工程已经创建了一组非天然的双功能氧化酶-过氧化物酶融合酶。人造酶具有结合两种必需辅因子的能力,并具有氧化酶和过氧化物酶的催化活性。这些融合酶可用于生物传感目的或用于双氧驱动的一锅两步级联氧化反应。
更新日期:2017-10-11
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