Creating Oxidase–Peroxidase Fusion Enzymes as a Toolbox for Cascade Reactions,ChemBioChem

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Creating Oxidase–Peroxidase Fusion Enzymes as a Toolbox for Cascade Reactions
ChemBioChem ( IF 2.6 ) Pub Date : 2017-10-11 06:25:20 , DOI: 10.1002/cbic.201700478
Dana I. Colpa ₁ , Nikola Lončar ₂ , Mareike Schmidt ₁ , Marco W. Fraaije ₁

Affiliation

Enzyme matchmaking: A set of unnatural bifunctional oxidase–peroxidases fusion enzymes have been created through protein engineering. The artificial enzymes retain the capability to bind the two required cofactors and are catalytically active as oxidases and peroxidases. These fusion enzymes can be used for biosensing purposes or for dioxygen-driven, one-pot, two-step cascade oxidation reactions.

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