Visualizing the Adenylation Activities and Protein–Protein Interactions of Aryl Acid Adenylating Enzymes,ChemBioChem

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Visualizing the Adenylation Activities and Protein–Protein Interactions of Aryl Acid Adenylating Enzymes
ChemBioChem ( IF 2.6 ) Pub Date : 2017-10-11 06:20:26 , DOI: 10.1002/cbic.201700361
Fumihiro Ishikawa ₁ , Shota Kasai ₂ , Hideaki Kakeya ₂ , Genzoh Tanabe ₁

Affiliation

Interacting nicely: Herein, we describe a new gel-based strategy coupled with activity-based probes to visualize and assess functional interactions, including protein–protein interactions, between aryl carrier protein (ArCP) and adenylation (A) domains as well as the substrate specificities of the A domains. This technique should facilitate biochemical, structural, and activity studies of highly specific, dynamic, and transient interactions.

中文翻译：

可视化芳酸腺苷酸化酶的腺苷酸化活性和蛋白-蛋白质相互作用

良好的交互作用：在此，我们描述了一种新的基于凝胶的策略以及基于活性的探针，以可视化并评估芳基载体蛋白（ArCP）和腺苷酸化（A）域以及底物之间的功能相互作用，包括蛋白质之间的相互作用A结构域的特异性。该技术应促进对高度特异性，动态和瞬时相互作用的生化，结构和活性研究。

更新日期：2017-10-11

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