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Chiral self-assembly of fullerene clusters on CT-DNA templates
Faraday Discussions ( IF 3.3 ) Pub Date : 2017-10-05 , DOI: 10.1039/c7fd00196g Sandeepa Kulala Vittala 1, 2, 3, 4, 5 , Joshy Joseph 1, 2, 3, 4, 5
Faraday Discussions ( IF 3.3 ) Pub Date : 2017-10-05 , DOI: 10.1039/c7fd00196g Sandeepa Kulala Vittala 1, 2, 3, 4, 5 , Joshy Joseph 1, 2, 3, 4, 5
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Herein we discuss the differential interaction of three monosubstituted fullerene derivatives possessing pyridinium, aniline or phenothiazine end groups (F-Py, F-An and F-PTz, respectively) with calf thymus DNA (CT-DNA), probed via spectroscopic and imaging techniques. The pyridinium derivative, F-Py becomes molecularly dissolved in 10% DMSO–PBS and interacts with CT-DNA via groove binding and electrostatic interactions, leading to the initial condensation of CT-DNA into micrometer sized aggregates and subsequent precipitation. On the other hand, the aniline derivative F-An, which is reported to form nanoclusters of 3–5 nm size, interacts with DNA through ordered, chiral assemblies on the CT-DNA template, thus perturbing the highly networked structure of CT-DNA to form nanonetworks, which eventually transform into condensed aggregates. The binding interactions between CT-DNA and F-An nanoclusters were established via UV-Vis, AFM and TEM analysis, and the chiral nature of the fullerene nanocluster assemblies on CT-DNA was confirmed by the presence of induced circular dichroism that was exhibited around the 250–370 nm region, corresponding to F-An nanocluster absorption. In contrast, the phenothiazine derivative F-PTz, which forms larger nanoclusters of ∼70 nm size in 10% DMSO–PBS, exhibited only weak interactions with CT-DNA without affecting its network structure. These results demonstrate the role of the hydrophobic–hydrophilic balance in the design of DNA interacting fullerene derivatives by controlling their cluster size and interactions with CT-DNA, and are significant in applications such as DNA condensation, gene delivery and dimension controlled nanomaterial fabrication.
中文翻译：
CT-DNA模板上富勒烯簇的手性自组装
在本文中，我们讨论了三种具有吡啶鎓，苯胺或吩噻嗪端基的单取代富勒烯衍生物（分别为F-Py，F-An和F-PTz）与小牛胸腺DNA（CT-DNA）的相互作用，通过光谱和成像技术进行了研究。 。吡啶鎓衍生物F-Py分子溶解在10％DMSO-PBS中，并通过凹槽结合和静电相互作用与CT-DNA相互作用，导致CT-DNA最初缩合成微米级的聚集体，随后沉淀。另一方面，苯胺衍生物F-An据报道，它形成3–5 nm的纳米簇，通过CT-DNA模板上的有序手性组装体与DNA相互作用，从而扰乱了CT-DNA的高度网络化结构，从而形成了纳米网络，最终转变为凝聚的聚集体。通过UV-Vis，AFM和TEM分析建立了CT-DNA与F-An纳米簇之间的结合相互作用，并通过在周围出现的诱导的圆二色性证实了富勒烯纳米簇组件在CT-DNA上的手性。 250-370 nm区域，对应于F-An纳米簇的吸收。相反，吩噻嗪衍生物F-PTz在10％DMSO-PBS中形成约70 nm的较大纳米簇，与CT-DNA的相互作用较弱，而不会影响其网络结构。这些结果证明了疏水-亲水平衡在设计与DNA相互作用的富勒烯衍生物中的作用，方法是控制它们的簇大小和与CT-DNA的相互作用，并且在诸如DNA缩合，基因传递和尺寸受控的纳米材料制造等应用中具有重要意义。
更新日期：2018-04-17
中文翻译：
CT-DNA模板上富勒烯簇的手性自组装
在本文中，我们讨论了三种具有吡啶鎓，苯胺或吩噻嗪端基的单取代富勒烯衍生物（分别为F-Py，F-An和F-PTz）与小牛胸腺DNA（CT-DNA）的相互作用，通过光谱和成像技术进行了研究。 。吡啶鎓衍生物F-Py分子溶解在10％DMSO-PBS中，并通过凹槽结合和静电相互作用与CT-DNA相互作用，导致CT-DNA最初缩合成微米级的聚集体，随后沉淀。另一方面，苯胺衍生物F-An据报道，它形成3–5 nm的纳米簇，通过CT-DNA模板上的有序手性组装体与DNA相互作用，从而扰乱了CT-DNA的高度网络化结构，从而形成了纳米网络，最终转变为凝聚的聚集体。通过UV-Vis，AFM和TEM分析建立了CT-DNA与F-An纳米簇之间的结合相互作用，并通过在周围出现的诱导的圆二色性证实了富勒烯纳米簇组件在CT-DNA上的手性。 250-370 nm区域，对应于F-An纳米簇的吸收。相反，吩噻嗪衍生物F-PTz在10％DMSO-PBS中形成约70 nm的较大纳米簇，与CT-DNA的相互作用较弱，而不会影响其网络结构。这些结果证明了疏水-亲水平衡在设计与DNA相互作用的富勒烯衍生物中的作用，方法是控制它们的簇大小和与CT-DNA的相互作用，并且在诸如DNA缩合，基因传递和尺寸受控的纳米材料制造等应用中具有重要意义。
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