Synthetic biology designed cell-free biosensors are a promising new tool for the detection of clinically relevant biomarkers in infectious diseases. Here, we report that a modular DNA-encoded biosensor in cell-free protein expression systems can be used to measure a bacterial biomarker of Pseudomonas aeruginosa infection from human sputum samples. By optimizing the cell-free system and sample extraction, we demonstrate that the quorum sensing molecule 3-oxo-C12-HSL in sputum samples from cystic fibrosis lungs can be quantitatively measured at nanomolar levels using our cell-free biosensor system, and is comparable to LC–MS measurements of the same samples. This study further illustrates the potential of modular cell-free biosensors as rapid, low-cost detection assays that can inform clinical practice.

中文翻译：

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一种用于检测铜绿假单胞菌感染的呼吸道样本中群体感应分子的无细胞生物传感器

合成生物学设计的无细胞生物传感器是一种很有前途的新工具，可用于检测传染病中临床相关的生物标志物。在这里，我们报告了无细胞蛋白表达系统中的模块化 DNA 编码生物传感器可用于测量人类痰液样本中铜绿假单胞菌感染的细菌生物标志物。通过优化无细胞系统和样品提取，我们证明使用我们的无细胞生物传感器系统可以在纳摩尔水平定量测量囊性纤维化肺痰样本中的群体感应分子 3-oxo-C12-HSL，并且具有可比性到相同样品的 LC-MS 测量。这项研究进一步说明了模块化无细胞生物传感器作为可以为临床实践提供信息的快速、低成本检测分析的潜力。