Abstract The polycondensation of a silane derivative such as aminopropyltrimethoxysilane (ATMS) in the presence of DNA was investigated in an organic solvent for the first time. Here, NMR observation showed that the hydrolysis step of ATMS before polycondensation was faster when the reaction was carried out in presence of double stranded DNA (146 bp) in chloroform. In order to test the specificity of this enhancement effect, the influence of the different components of DNA on ATMS hydrolysis and/or polycondensation was then investigated using different bases, nucleosides and nucleotides. The results showed that the kinetics of ATMS hydrolysis was affected by the type of nucleotide units used, the faster hydrolysis reaction rate being observed with molecules containing adenosine group, and that in the absence of water the amino group of deoxyadenosine units can react with ATMS methoxy groups.

中文翻译：

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氨丙基三甲氧基硅烷在 DNA 上的模板缩聚反应

摘要 首次在有机溶剂中研究了氨基丙基三甲氧基硅烷 (ATMS) 等硅烷衍生物在 DNA 存在下的缩聚反应。在这里，核磁共振观察表明，当反应在双链 DNA (146 bp) 的氯仿存在下进行时，缩聚前的 ATMS 水解步骤更快。为了测试这种增强效应的特异性，然后使用不同的碱基、核苷和核苷酸研究了 DNA 的不同成分对 ATMS 水解和/或缩聚的影响。结果表明，ATMS水解的动力学受所用核苷酸单元类型的影响，含腺苷基团的分子水解反应速度较快，