Fluorescent Glyco Single-Chain Nanoparticle-Decorated Nanodiamonds,ACS Macro Letters

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Fluorescent Glyco Single-Chain Nanoparticle-Decorated Nanodiamonds
ACS Macro Letters ( IF 5.1 ) Pub Date : 2017-10-04 00:00:00 , DOI: 10.1021/acsmacrolett.7b00659
Kilian N R Wuest _{1,

2} , Hongxu Lu ₂ , Donald S Thomas ₃ , Anja S Goldmann _{1,

4} , Martina H Stenzel ₂ , Christopher Barner-Kowollik _{1,

4}

Affiliation

We introduce the light-induced collapse of single glycopolymer chains in water generating fluorescent glyco single-chain nanoparticles (SCNPs) and their subsequent functionalization onto nanodiamonds. The glycopolymer precursors are prepared by polymerizing an acetylated mannose-based methacrylate monomer followed by a deprotection and postpolymerization functionalization step, introducing profluorescent photoactive tetrazole groups and furan-protected maleimide moieties. Subsequent UV irradiation in highly diluted aqueous solution triggers intramolecular tetrazole-mediated cycloadditions, yielding glyco SCNPs featuring fluorescence as well as lectin binding properties. The obtained SCNPs are coated onto nanodiamonds by adsorption, and the obtained hybrid nanoparticles are in depth characterized in terms of size, functionality, and bioactivity. Different coating densities are achieved by altering the SCNP concentration. The prepared nanoparticles are nontoxic in mouse RAW 264.7 macrophages. Furthermore, the fluorescence of the SCNPs can be exploited to image the SCNP-coated nanodiamonds in macrophage cells via confocal fluorescence microscopy.

中文翻译：

荧光糖单链纳米颗粒装饰的纳米金刚石

我们介绍了水生成荧光糖单链纳米粒子 (SCNP) 中单个糖聚合物链的光诱导塌陷及其随后在纳米金刚石上的功能化。糖聚合物前体是通过聚合乙酰化甘露糖基甲基丙烯酸酯单体，然后进行脱保护和后聚合功能化步骤，引入荧光光活性四唑基团和呋喃保护的马来酰亚胺部分来制备的。随后在高度稀释的水溶液中进行紫外线照射会触发分子内四唑介导的环加成反应，产生具有荧光和凝集素结合特性的糖类 SCNP。将获得的 SCNPs 通过吸附包覆在纳米金刚石上，并在尺寸、功能和生物活性方面对获得的杂化纳米颗粒进行深入表征。通过改变 SCNP 浓度可以获得不同的涂层密度。制备的纳米颗粒对小鼠 RAW 264.7 巨噬细胞无毒。此外，可以利用 SCNP 的荧光通过共聚焦荧光显微镜对巨噬细胞中的 SCNP 涂层纳米金刚石进行成像。

更新日期：2017-10-04

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