In this study, essential oil (EO) nanoemulsions as an alternative to chlorine-based sanitizers for red mustard leaves were prepared, and their inhibitory effects against pathogenic bacteria were examined. Red mustard leaves spot-inoculated with Listeria monocytogenes, Escherichia coli O157:H7, and Salmonella Typhimurium were treated with EOs alone, surfactants (SFs) (Tween 80 (T80), non-ionic; cetylpyridinium chloride (CPC), cationic) alone, and nanoemulsions formulated with EOs (0.02%) and SFs (0.002%). The results indicated that nanoemulsions containing CPC exhibited a higher inhibitory effect than those including T80. The reductions in all pathogens after treatment with nanoemulsions containing EOs and CPC were higher (0.1–0.6 log reductions) than those of 0.02% NaOCl. In addition, nanoemulsion treatment did not affect the sensory qualities of red mustard during storage. Therefore, nanoemulsions with EOs and CPC can become an effective substitute for chlorine-based sanitizers for fresh-cut produce if regulations allow the use of CPC.

Industrial relevance

Foodborne-illness outbreaks related to fresh-cut produce have increased globally. The use of chlorine-based sanitizers in the fresh-cut produce industry has been decreasing due to health-related problems. Thus, an alternative washing treatment is needed to control pathogenic bacteria contaminating fresh-cut produce. Nanoemulsions with EOs and cationic SF developed in this study can be a suitable washing agent for inactivating foodborne pathogens on fresh-cut produce.

中文翻译：

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植物精油纳米乳剂对单核细胞增生李斯特菌，大肠杆菌O157：H7和鼠伤寒沙门氏菌的抑制作用

在这项研究中，制备了香精油（EO）纳米乳液，作为芥菜红叶的氯基消毒剂的替代品，并研究了它们对病原菌的抑制作用。点染李斯特菌，大肠杆菌O157：H7和沙门氏菌的芥菜红叶鼠伤寒单独用EO，表面活性剂（SFs）（Tween 80（T80），非离子型;十六烷基氯化吡啶鎓（CPC），阳离子）单独处理，并用EOs（0.02％）和SFs（0.002％）配制纳米乳液。结果表明，含有CPC的纳米乳剂比含有T80的纳米乳剂具有更高的抑制作用。用含有EOs和CPC的纳米乳剂处理后，所有病原体的减少量均高于0.02％NaOCl的减少量（减少0.1–0.6 log）。另外，纳米乳液处理在储存过程中不影响红芥末的感官品质。因此，如果法规允许使用CPC，则具有EOs和CPC的纳米乳液可以成为鲜切农产品的氯基消毒剂的有效替代品。

行业相关性

与鲜切农产品有关的食源性疾病暴发在全球范围内有所增加。由于健康相关的问题，鲜切农产品行业中氯基消毒剂的使用量一直在减少。因此，需要替代的洗涤处理以控制污染鲜切农产品的病原细菌。这项研究中开发的具有EOs和阳离子SF的纳米乳剂可以用作灭活鲜切食品中食源性病原体的合适洗涤剂。