The accurate detection of disease-related biomarkers is crucial for the early diagnosis and management of disease in personalized medicine. Here, we present a molecular imaging of human epidermal growth factor receptor (EGFR)-expressing malignant tumors using an EGFR-specific repebody composed of leucine-rich repeat (LRR) modules. The repebody was labeled with either a fluorescent dye or radioisotope, and used for imaging of EGFR-expressing malignant tumors using an optical method and positron emission tomography. Our approach enabled visualization of the status of EGFR expression, allowing quantitative evaluation in whole tumors, which correlated well with the EGFR expression levels in mouse or patients-derived colon cancers. The present approach can be effectively used for the accurate detection of EGFR-expressing cancers, assisting in the development of a tool for detecting other disease biomarkers.

中文翻译：

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高亲和性抗体表达EGFR表达的恶性肿瘤的分子成像。

疾病相关生物标记物的准确检测对于个性化医学中疾病的早期诊断和管理至关重要。在这里，我们介绍了人类表皮生长因子受体（EGFR）表达的恶性肿瘤的分子成像，使用由富含亮氨酸的重复序列（LRR）模块组成的EGFR特异性复制抗体。用荧光染料或放射性同位素标记重标抗体，并使用光学方法和正电子发射断层扫描术对表达EGFR的恶性肿瘤进行成像。我们的方法能够观察到EGFR表达的状态，从而可以对整个肿瘤进行定量评估，这与小鼠或患者来源的结肠癌中EGFR表达水平高度相关。本方法可有效用于准确检测表达EGFR的癌症，