Development of Antifouling and Bactericidal Coatings for Platelet Storage Bags Using Dopamine Chemistry,Advanced Healthcare Materials

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Development of Antifouling and Bactericidal Coatings for Platelet Storage Bags Using Dopamine Chemistry
Advanced Healthcare Materials ( IF 10.0 ) Pub Date : 2017-09-29 , DOI: 10.1002/adhm.201700839
Narges Hadjesfandiari _{1,

2} , Marie Weinhart ₃ , Jayachandran N. Kizhakkedathu _{1,

2,

4} , Rainer Haag ₃ , Donald E. Brooks _{1,

2,

4}

Affiliation

Platelets have a limited shelf life, due to the risk of bacterial contamination and platelet quality loss. Most platelet storage bags are made of a mixture of polyvinyl chloride with a plasticizer, denoted as pPVC. To improve biocompatibility of pPVC with platelets and to inhibit bacterial biofilm formation, an antifouling polymer coating is developed using mussel‐inspired chemistry. A copolymer of N,N‐dimethylacrylamide and N‐(3‐aminopropyl)methacrylamide hydrochloride is synthesized and coupled with catechol groups, named DA51‐cat. Under mild aqueous conditions, pPVC is first equilibrated with an anchoring polydopamine layer, followed by a DA51‐cat layer. Measurements show this coating decreases fibrinogen adsorption to 5% of the control surfaces. One‐step coating with DA51‐cat does not coat pPVC efficiently although it is sufficient for coating silicon wafers and gold substrates. The dual layer coating on platelet bags resists bacterial biofilm formation and considerably decreases platelet adhesion. A cationic antimicrobial peptide, E6, is conjugated to DA51‐cat then coated on silicon wafers and introduces bactericidal activity to these surfaces. Time‐of‐flight second ion‐mass spectroscopy is successfully applied to characterize these surfaces. pPVC is widely used in medical devices; this method provides an approach to controlling biofouling and bacterial growth on it without elaborate surface modification procedures.

中文翻译：

用多巴胺化学技术开发用于血小板存储袋的防污和杀菌涂层

由于存在细菌污染和血小板质量损失的风险，血小板的保质期有限。大多数血小板存储袋由聚氯乙烯和增塑剂（称为pPVC）的混合物制成。为了提高pPVC与血小板的生物相容性并抑制细菌生物膜的形成，使用贻贝启发化学技术开发了一种防污聚合物涂层。N，N-二甲基丙烯酰胺和N的共聚物合成了（3-氨基丙基）甲基丙烯酰胺盐酸盐，并与名为DA51-cat的儿茶酚基团偶联。在温和的水性条件下，pPVC首先通过锚固聚多巴胺层，然后是DA51-cat层进行平衡。测量表明，该涂层将纤维蛋白原的吸附量降低至对照表面的5％。用DA51-cat进行的一步涂覆不能有效地涂覆pPVC，尽管它足以涂覆硅晶片和金衬底。血小板袋上的双层涂层可抵抗细菌生物膜的形成，并显着降低血小板的附着力。阳离子抗菌肽E6与DA51-cat偶联，然后涂在硅片上，并向这些表面引入杀菌活性。飞行时间二次离子质谱已成功应用于表征这些表面。pPVC广泛用于医疗设备；该方法提供了一种无需复杂的表面改性程序即可控制生物污垢和细菌在其上生长的方法。

更新日期：2017-09-29

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