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Development of a Scalable and Sustainable High Performance CounterCurrent Chromatography (HPCCC) Purification for Spinosyn A and Spinosyn D from Spinosad
Organic Process Research & Development ( IF 3.1 ) Pub Date : 2017-09-29 00:00:00 , DOI: 10.1021/acs.oprd.7b00249 Carl DeAmicis 1 , Qiang Yang 1 , Colin Bright 2 , Neil A. Edwards 2 , Guy H. Harris 2 , Sandeep Kaur 2 , Philip L. Wood 2 , Peter Hewitson 3 , Svetlana Ignatova 3
Organic Process Research & Development ( IF 3.1 ) Pub Date : 2017-09-29 00:00:00 , DOI: 10.1021/acs.oprd.7b00249 Carl DeAmicis 1 , Qiang Yang 1 , Colin Bright 2 , Neil A. Edwards 2 , Guy H. Harris 2 , Sandeep Kaur 2 , Philip L. Wood 2 , Peter Hewitson 3 , Svetlana Ignatova 3
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A high-performance countercurrent chromatography (HPCCC) process was developed as a more efficient and sustainable alternative to reverse phase high-performance liquid chromatography (RP-HPLC) for the pilot-scale purification of the naturally occurring fermentation-derived insecticides, spinosyn A and spinosyn D, the major components of spinosad insecticide. While on pilot scale HPCCC and RP-HPLC both gave >99% purities and comparable combined recoveries of 77% and 83%, respectively, HPCCC was much more efficient and sustainable by producing a 60% higher productivity, 11 times higher solute loading, 96% savings in stationary phase costs, and 42% reduction in solvent usage. The increase in productivity and reduction in solvent usage further reduced waste recycle and disposal costs, thus presenting significantly less environmental impact compared to RP-HPLC separations. The use of mixing on demand for a solvent system at the preparative scale allowed a complete automation with minimized solvent consumption.
中文翻译：
衍生自Spinosad的Spinosyn A和Spinosyn D的可扩展且可持续的高性能逆流色谱（HPCCC）纯化的开发
开发了一种高效逆流色谱（HPCCC）工艺，可以更高效，更可持续地替代反相高效液相色谱（RP-HPLC），用于中试规模纯化天然发酵衍生的杀虫剂，多杀菌素A和多杀菌素A。 spinosyn D，多杀菌素杀虫剂的主要成分。在中试规模下，HPCCC和RP-HPLC的纯度分别> 99％，可比的组合回收率分别为77％和83％，HPCCC的生产率提高了60％，溶质负载提高了11倍，效率和可持续性更高，96节省了固定相成本的百分比，并减少了42％的溶剂使用量。生产率的提高和溶剂用量的减少进一步减少了废物回收和处置成本，因此，与RP-HPLC分离相比，对环境的影响要小得多。在制备规模上按需混合使用溶剂系统可实现完全自动化，同时溶剂消耗量降至最低。
更新日期：2017-09-30
中文翻译：
衍生自Spinosad的Spinosyn A和Spinosyn D的可扩展且可持续的高性能逆流色谱（HPCCC）纯化的开发
开发了一种高效逆流色谱（HPCCC）工艺，可以更高效，更可持续地替代反相高效液相色谱（RP-HPLC），用于中试规模纯化天然发酵衍生的杀虫剂，多杀菌素A和多杀菌素A。 spinosyn D，多杀菌素杀虫剂的主要成分。在中试规模下，HPCCC和RP-HPLC的纯度分别> 99％，可比的组合回收率分别为77％和83％，HPCCC的生产率提高了60％，溶质负载提高了11倍，效率和可持续性更高，96节省了固定相成本的百分比，并减少了42％的溶剂使用量。生产率的提高和溶剂用量的减少进一步减少了废物回收和处置成本，因此，与RP-HPLC分离相比，对环境的影响要小得多。在制备规模上按需混合使用溶剂系统可实现完全自动化，同时溶剂消耗量降至最低。
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