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Phylogeny of xerophilic aspergilli (subgenus Aspergillus) and taxonomic revision of section Restricti
Studies in Mycology ( IF 14.1 ) Pub Date : 2017-09-27 , DOI: 10.1016/j.simyco.2017.09.002
F. Sklenář , Ž. Jurjević , P. Zalar , J.C. Frisvad , C.M. Visagie , M. Kolařík , J. Houbraken , A.J. Chen , N. Yilmaz , K.A. Seifert , M. Coton , F. Déniel , N. Gunde-Cimerman , R.A. Samson , S.W. Peterson , V. Hubka

Aspergillus section Restricti together with sister section Aspergillus (formerly Eurotium) comprises xerophilic species, that are able to grow on substrates with low water activity and in extreme environments. We adressed the monophyly of both sections within subgenus Aspergillus and applied a multidisciplinary approach for definition of species boundaries in sect. Restricti. The monophyly of sections Aspergillus and Restricti was tested on a set of 102 isolates comprising all currently accepted species and was strongly supported by Maximum likelihood (ML) and Bayesian inferrence (BI) analysis based on β-tubulin (benA), calmodulin (CaM) and RNA polymerase II second largest subunit (RPB2) loci. More than 300 strains belonging to sect. Restricti from various isolation sources and four continents were characterized by DNA sequencing, and 193 isolates were selected for phylogenetic analyses and phenotypic studies. Species delimitation methods based on multispecies coalescent model were employed on DNA sequences from four loci, i.e., ID region of rDNA (ITS + 28S), CaM, benA and RPB2, and supported recognition of 21 species, including 14 new. All these species were also strongly supported in ML and BI analyses. All recognised species can be reliably identified by all four examined genetic loci. Phenotype analysis was performed to support the delimitation of new species and includes colony characteristics on seven cultivation media incubated at several temperatures, growth on an osmotic gradient (six media with NaCl concentration from 0 to 25 %) and analysis of morphology including scanning electron microscopy. The micromorphology of conidial heads, vesicle dimensions, temperature profiles and growth parameters in osmotic gradient were useful criteria for species identification.

The vast majority of species in sect. Restricti produce asperglaucide, asperphenamate or both in contrast to species in sect. Aspergillus. Mycophenolic acid was detected for the first time in at least six members of the section. The ascomata of A. halophilicus do not contain auroglaucin, epiheveadride or flavoglaucin which are common in sect. Aspergillus, but shares the echinulins with sect. Aspergillus.



中文翻译:

嗜干曲霉(亚曲霉属)的系统发育和Restricti节的分类学修订。

限制曲霉部分和姊妹部分曲霉(以前称为Euro)包含嗜干菌种,它们能够在水分活度低且在极端环境下生长的底物上生长。我们研究了曲霉属下两个部分的单性,并应用了多学科方法来定义该物种中的物种边界。限制。部分的单系曲霉Restricti是一组102个菌株,包括所有目前公认的物种测试并通过最大似然(ML)和贝叶斯inferrence(BI)分析基于β微管蛋白的强有力支持(笨啊),钙调蛋白(CaM)和RNA聚合酶II第二大亚基(RPB2)位点。属于该教派的菌株超过300种。通过DNA测序来自不同分离源和四大洲的限制区进行了鉴定,并选择了193个分离区进行系统发育分析和表型研究。对来自四个基因座的DNA序列采用基于多物种合并模型的物种定界方法,即rDNA的ID区域(ITS + 28S),CaMbenARPB2,并支持识别21种,其中包括14种。所有这些种类在ML和BI分析中也得到了强有力的支持。可以通过所有四个检查的遗传基因座可靠地识别所有公认的物种。进行表型分析以支持新物种的定界,包括在几种温度下孵育的七种培养基上的菌落特征,在渗透梯度上生长(六种NaCl浓度从0%到25%的培养基)以及包括扫描电子显微镜在内的形态分析。分生孢子头的微观形态,囊泡尺寸,温度分布和渗透梯度中的生长参数是进行物种鉴定的有用标准。

该教派中绝大多数物种。与该种类的物种相比,限制类产生曲霉葡糖苷,曲霉芬酸酯或两者兼而有之。曲霉。在该部分的至少六个成员中首次检测到麦考酚酸。嗜盐气单胞的子囊不包含在该领域中常见的金绿霉素,表阿维德胺或黄霉素。曲霉菌,但与该菌种共享肌钙蛋白。曲霉

更新日期:2017-09-27
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