Over the years mainly three unconventional fluorescence techniques, Excitation-emission matrix fluorescence (EEMF), synchronous fluorescence spectroscopy (SFS), and total synchronous fluorescence spectroscopy (TSFS) are introduced for the analysis of multifluorophoric mixtures. Application of EEMF, SFS and TSFS are conceptually different. The existing literature lacks a review article that gives an overview on conceptual and analytical aspects of EEMF, SFS and TSFS for general as well as specialized fluorescence scientific community. The present review article attempts to address these issues and discusses various conceptual and practical aspects of EEMF, SFS and TSFS spectroscopy. The present article contains numerous novel fluorescence parameters, concept of concentration dependent red shift, protocol for finding the optimum wavelength offset for SFS data acquisition is introduced, various practical aspects of integrating chemometric methods with TSFS and number of successful applications of EEMF, SFS and TSFS for the analysis of complex and simple multifluorophoric mixtures is discussed.

中文翻译：

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非常规稳态荧光光谱作为一种分析技术，用于分析复杂的多荧光团混合物

多年来，主要介绍三种非常规荧光技术，即激发发射矩阵荧光（EEMF），同步荧光光谱（SFS）和总同步荧光光谱（TSFS）来分析多荧光混合物。EEMF，SFS和TSFS的应用在概念上有所不同。现有文献缺乏评论文章，概述了针对通用以及专门的荧光科学界的EEMF，SFS和TSFS的概念和分析方面。本篇综述文章试图解决这些问题，并讨论了EEMF，SFS和TSFS光谱学的各种概念和实践方面。本文包含许多新颖的荧光参数，浓度依赖性红移的概念，