Vaccinia virus (VACV), a member of the Poxviridae family, uses cytoplasmic factories for its replication. Recent studies indicated that VACV infection requires a set of nucleoporins. However, how the nucleoporins contribute to viral life cycle remains unclear. Here, we report that the nucleoporins Nup62 and Nup358 localize to the cytoplasmic viral factories (VFs). Nup358 was targeted to the VFs at 6 h post-infection (hpi), whereas Nup62, along with the previously reported translation factors such as eIF4E, eIF3η and G3BP1, was recruited to the VFs at 8 hpi. Nup358 depletion led to a decrease in the size and number of viral factories and reduction in viral yield. Further studies showed that Nup358 is involved in recruiting Nup62 and eIF4E to the VFs. Collectively, our results reveal spatio-temporal regulation in the recruitment of nucleoporins and translation factors to VFs, and particularly the importance of Nup358 in VACV infection.

牛痘病毒（VACV），所述的构件痘病毒家族，使用细胞质工厂进行复制。最近的研究表明，VACV感染需要一组核孔蛋白。然而，尚不清楚核孔蛋白如何促进病毒生命周期。在这里，我们报告说，核孔蛋白Nup62和Nup358定位到细胞质病毒工厂（VFs）。Nup358在感染后6 h（hpi）靶向VF，而Nup62以及先前报道的翻译因子（例如eIF4E，eIF3η和G3BP1）在8 hpi被募集到VF。Nup358耗竭导致病毒工厂的大小和数量减少以及病毒产量降低。进一步的研究表明，Nup358参与了向VF募集Nup62和eIF4E。总的来说，我们的研究结果揭示了在向VFs募集核孔蛋白和翻译因子时的时空调控，