We have studied how simultaneously elevated temperature and CO₂ levels [climate change-related conditions (CCC) of 30 °C, 970 parts-per-million (ppm) of CO₂ vs. standard conditions (SC) of 25 °C, ~ 405 ppm CO₂] affect physiochemical properties of Nicotiana benthamiana leaves, and also its infection by several positive-sense RNA viruses. In previous works we had studied effects of elevated temperature, CO₂ levels separately. Under CCC, leaves of healthy plants almost doubled their area relative to SC but contained less protein/unit-of-area, similarly to what we had found under conditions of elevated CO₂ alone. CCC also affected the sizes/numbers of different foliar cell types differently. Under CCC, infection outcomes in titers and symptoms were virus type-specific, broadly similar to those observed under elevated temperature alone. Under either condition, infections did not significantly alter the protein content of leaf discs. Therefore, effects of elevated temperature and CO₂ combined on properties of the pathosystems studied were overall cumulative.

我们研究了如何同时升高温度和 CO ₂水平[气候变化相关条件 (CCC) 30 °C，CO ₂为百万分之 970 (ppm) 与标准条件 (SC) 25 °C，~ 405 ppm CO ₂ ]影响本塞姆氏烟草叶片的理化性质，也影响几种正链RNA病毒的感染。在之前的工作中，我们分别研究了高温、CO ₂水平的影响。在 CCC 条件下，健康植物的叶子面积相对于 SC 几乎增加了一倍，但每单位面积含有较少的蛋白质，与我们在单独升高 CO ₂的条件下发现的情况类似。 CCC 还对不同叶细胞类型的大小/数量产生不同的影响。在 CCC 下，滴度和症状的感染结果是病毒类型特异性的，与单独高温下观察到的结果大致相似。在任何一种情况下，感染都不会显着改变叶盘的蛋白质含量。因此，升高的温度和CO ₂结合对所研究的病理系统特性的影响是总体累积的。