Whole-Genome Shotgun Sequencing of Two β-Proteobacterial Species in Search of the Bulgecin Biosynthetic Cluster,ACS Chemical Biology

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Whole-Genome Shotgun Sequencing of Two β-Proteobacterial Species in Search of the Bulgecin Biosynthetic Cluster
ACS Chemical Biology ( IF 3.5 ) Pub Date : 2017-09-26 00:00:00 , DOI: 10.1021/acschembio.7b00687
Mark E. Horsman , Daniel R. Marous , Rongfeng Li ₁ , Ryan A. Oliver ₁ , Byungjin Byun , Scott J. Emrich , Bill Boggess , Craig A. Townsend ₁ , Shahriar Mobashery

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We have produced draft whole-genome sequences for two bacterial strains reported to produce the bulgecins as well as NRPS-derived monobactam β-lactam antibiotics. We propose classification of ATCC 31363 as Paraburkholderia acidophila. We further reaffirm that ATCC 31433 (Burkholderia ubonensis subsp. mesacidophila) is a taxonomically distinct producer of bulgecins with notable gene regions shared with Paraburkholderia acidophila. We use RAST multiple-gene comparison and MASH distancing with published genomes to order the draft contigs and identify unique gene regions for characterization. Forty-eight natural-product gene clusters are presented from PATRIC (RASTtk) and antiSMASH annotations. We present evidence that the 10 genes that follow the sulfazecin and isosulfazecin pathways in both species are likely involved in bulgecin A biosynthesis.

中文翻译：

寻找Bulgecin生物合成簇的两种β-Proteobacterial物种的全基因组Shot弹枪测序。

我们已经为两个细菌菌株生产了全基因组序列草稿，据报道它们产生了球蛋白和NRPS衍生的单杆菌素β-内酰胺类抗生素。我们建议将ATCC 31363归类为嗜酸羊角菌。我们进一步重申，ATCC 31433（布克霍尔德菌ubonensis亚种嗜酸菌）是一种与球菌素在分类学上截然不同的生产者，具有明显的基因区域与嗜酸巴拉伯氏菌共有。我们使用RAST多基因比较和已发布基因组的MASH距离来排序重叠群并确定用于表征的独特基因区域。从PATRIC（RASTtk）和antiSMASH注释中提出了48个天然产物基因簇。我们目前的证据表明，在两个物种中遵循磺胺ze呤和异磺胺ze嗪途径的10个基因可能参与了bulgecin A的生物合成。

更新日期：2017-09-26

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