As an important intermediate feedstock, succinate is termed as one of the 12 bio-based platform chemicals. To improve its fermentative production, various strategies have been developed, but challenges are still ahead for succinate biosynthesis to be cost-competitive. In this article, strategies for succinate production through metabolic engineering of Escherichia coli are critically reviewed, with a focus on engineering by-product formation and CO₂ fixation, substrate utilization, reducing power balance, metabolic evolution and transcriptional regulation, which provide insights for the current state of succinate production and perspectives for further research for more efficient and economical production of bio-based succinate.

作为重要的中间原料，琥珀酸酯被称为12种生物基平台化学品之一。为了提高其发酵生产，已经开发了各种策略，但是琥珀酸生物合成在成本上具有竞争力仍然面临挑战。在本文中，对通过大肠杆菌的代谢工程生产琥珀酸酯的策略进行了严格审查，重点是工程副产物的形成和CO ₂固定，底物利用，降低功率平衡，代谢进化和转录调控，这些都为我们提供了见识。琥珀酸酯生产的现状和进一步研究的前景，以更有效和经济地生产生物基琥珀酸酯。