Evidence supports that a high dietary fat intake increases oxidative stress and the risk of diet-induced metabolic disorders such as obesity, diabetes and cardiovascular diseases. F₂-isoprostanes (F₂-isoP) are formed by the non-enzymatic oxidation of arachidonic acid and are widely used as reliable biomarkers of oxidative stress in clinical studies. Dietary fats may influence F₂-isoP levels, as they (1) are metabolic substrates for their formation, (2) modify the lipid composition of tissues, and (3) affect the plasma lipoprotein concentrations which are involved in F₂-isoP transport. This review examined the latest clinical evidence on how dietary fats can affect blood circulation and excretion of F₂-isoP in individuals with healthy or deteriorated metabolic profiles. Clinical studies reported that saturated or monounsaturated fat-rich diets did not affect F₂-isoP levels in adults with healthy or deteriorated metabolic profiles. Though, ω-3 polyunsaturated fatty acids decreased F₂-isoP levels in numerous studies, whereas trans-fatty acids raised F₂-isoP excretion. Yet, the reported heterogeneous results reveal important considerations, such as the health status of the participants, the biological fluids used to determine F₂-isoP, the analytical methods employed and the specific F₂-isoP isomers detected. Therefore, future clinical studies should be designed in order to consider these issues in the studies of the effects of fat intake on oxidative stress.

有证据表明，高饮食脂肪摄入会增加氧化应激，并增加饮食引起的代谢性疾病（如肥胖症，糖尿病和心血管疾病）的风险。F _2-异前列腺素（F ₂ -isoP）是通过花生四烯酸的非酶氧化形成的，在临床研究中被广泛用作氧化应激的可靠生物标志物。膳食脂肪可能会影响F ₂ -isoP水平，因为它们（1）是其形成的代谢底物，（2）改变组织的脂质组成，并且（3）影响F ₂ -isoP转运所涉及的血浆脂蛋白浓度。这篇评论检查了有关饮食脂肪如何影响血液循环和F ₂排泄的最新临床证据。代谢状况健康或恶化的个体中的-isoP。临床研究报告说，饱和或单不饱和脂肪丰富的饮食不影响具有健康或不良代谢特征的成年人的F ₂ -isoP水平。但是，在许多研究中，ω-3多不饱和脂肪酸降低了F ₂ -isoP的水平，而反式脂肪酸则增加了F ₂ -isoP的排泄。但是，报告的异质性结果显示出重要的考虑因素，例如参与者的健康状况，用于确定F ₂ -isoP的生物液体，采用的分析方法和特定的F ₂检测到-isoP异构体。因此，应设计未来的临床研究，以便在脂肪摄入对氧化应激影响的研究中考虑这些问题。