Mass spectrometry is a highly complex analytical technique and mass spectrometry‐based proteomics experiments can be subject to a large variability, which forms an obstacle to obtaining accurate and reproducible results. Therefore, a comprehensive and systematic approach to quality control is an essential requirement to inspire confidence in the generated results. A typical mass spectrometry experiment consists of multiple different phases including the sample preparation, liquid chromatography, mass spectrometry, and bioinformatics stages. We review potential sources of variability that can impact the results of a mass spectrometry experiment occurring in all of these steps, and we discuss how to monitor and remedy the negative influences on the experimental results. Furthermore, we describe how specialized quality control samples of varying sample complexity can be incorporated into the experimental workflow and how they can be used to rigorously assess detailed aspects of the instrument performance.

中文翻译：

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基于质谱的蛋白质组学的质量控制

质谱分析是一种非常复杂的分析技术，基于质谱的蛋白质组学实验可能会有很大的差异，这对获得准确和可重复的结果构成了障碍。因此，全面而系统的质量控制方法是激发人们对所产生结果的信心的基本要求。典型的质谱实验由多个不同阶段组成，包括样品制备，液相色谱，质谱和生物信息学阶段。我们回顾了可能影响所有这些步骤中发生的质谱实验结果的可变性来源，并讨论了如何监视和纠正对实验结果的负面影响。此外，