In single‐molecule mass spectrometry, the mass of each ion is measured individually; making it suitable for the analysis of very large, heterogeneous objects that cannot be analyzed by conventional means. A range of single‐molecule mass spectrometry techniques has been developed, including time‐of‐flight with cryogenic detectors, a quadrupole ion trap with optical detection, single‐molecule Fourier transform ion cyclotron resonance, charge detection mass spectrometry, quadrupole ion traps coupled to charge detector plates, and nanomechanical oscillators. In addition to providing information on mass and heterogeneity, these techniques have been used to study impact craters from cosmic dust, monitor the assembly of viruses, elucidate the fluorescence dynamics of quantum dots, and much more. This review focuses on the merits of each of these technologies, their limitations, and their applications. © 2016 Wiley Periodicals, Inc. Mass Spec Rev 36:715–733, 2017

中文翻译：

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单分子质谱

在单分子质谱法中，每个离子的质量分别测量。使其适用于无法通过常规方法分析的非常大的异构对象的分析。已经开发了一系列单分子质谱技术，包括具有低温检测器的飞行时间，具有光学检测功能的四极离子阱，单分子傅里叶变换离子回旋共振，电荷检测质谱，与四极杆耦合的四极离子阱电荷检测器板和纳米机械振荡器。除了提供有关质量和异质性的信息外，这些技术还用于研究宇宙尘埃产生的撞击坑，监视病毒的组装，阐明量子点的荧光动力学等等。本文将重点介绍每种技术的优缺点，局限性及其应用。分级为4 +©2016 Wiley Periodicals，Inc.Mass Spec Rev 36：715–733，2017