Atmospheric cold plasma is a green and emerging technology, highly interesting to the food industry for its application. Dielectric Barrier Discharges (DBD) can generate atmospheric cold plasma inside sealed packages filled with air through the use of high voltages. This study investigated the use of a large gap DBD design to generate a plasma discharge within the headspace of packaged herring fillets, and its effects on microbiological and quality markers of the fish stored for 11 days at 4 °C. DBD plasma treatment conditions were 70 kV or 80 kV for 5 min treatment time.

Results showed that the microbial load (total aerobic mesophilic, total aerobic psychrotrophics, Pseudomonas, lactic acid bacteria and Enterobacteriaceae) were significantly (p < 0.05) lower in the treated samples compared to the control samples. However, samples exposed to the lowest applied voltage better retained key quality factors (lower oxidation and less colour modification). DBD-treatment caused a reduction in trapped water in the myofibrillar network (T21), identified using low-field nuclear magnetic resonance of protons (¹H LF NMR).

The results indicate that in-package plasma treatment could be employed as an effective treatment for reducing spoilage bacteria in fish.

Industrial relevance

Dielectric Barrier Discharge (DBD) was evaluated as a treatment for highly perishable fish products. DBD's is a safety and lower costs alternative for processing industry. DBD has been scarcely tested on fish; this work showed results on quality and shelf-life of a highly perishable fish species, which might serve as reference for processing optimisation of fish products minimally processed by DBD.

中文翻译：

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使用包装内大气等离子体技术延长鲱鱼（Clupea harengus）的保质期

大气冷等离子体是一种绿色的新兴技术，对于食品工业的应用非常有趣。介质阻挡放电（DBD）可以通过使用高压在充满空气的密封包装内产生大气冷等离子体。这项研究调查了使用大间隙DBD设计在包装的鲱鱼片的顶部空间内产生等离子体放电的情况，及其对在4°C下储存11天的鱼的微生物学和质量标记的影响。DBD等离子体处理条件为70 kV或80 kV，处理时间为5分钟。

结果表明，与对照样品相比，处理后的样品中的微生物负荷（总有氧嗜温性，总有氧精神营养性，假单胞菌，乳酸菌和肠杆菌科）显着降低（p <0.05）。但是，暴露在最低施加电压下的样品可以更好地保留关键的品质因数（较低的氧化作用和较少的颜色修饰）。使用质子的低场核磁共振（¹ H LF NMR）可以确认，DBD处理导致肌原纤维网络（T21）中截留的水减少。

结果表明，包装内等离子体处理可以作为减少鱼类腐败细菌的有效方法。

行业相关性

介电屏障放电（DBD）被评估为高度易腐烂鱼产品的处理方法。对于加工行业而言，DBD是一种安全且成本较低的替代品。DBD几乎没有在鱼类上进行过测试；这项工作显示了关于高度易腐烂鱼类的质量和保质期的结果，这可以为DBD最少加工的鱼制品的加工优化提供参考。