Enterobacter sp. was grown on glycerol byproduct from the biodiesel industry for the production of a value-added exopolysaccharide (EPS). The culture broth was characterized in terms of its morphological and rheological properties throughout the cultivation run. Microscopic observations revealed the formation of cell aggregates surrounded by the EPS at the beginning of the cultivation run, while, at the end, aggregates were reduced and an EPS matrix with the cells embedded in it was observed. The apparent viscosity of the culture broth increased over time, which was attributed to the increase of the EPS concentration in the first period of the cultivation run. However, in the final stage, the creation of new polymer interactions within the complex culture broth was likely the reason for the viscosity increase observed, since there was not a significant variation of the EPS concentration, average molecular weight or chemical composition. The broth presented a Newtonian behavior at the beginning of the run, changing to pseudoplastic as the EPS concentration increased, and revealed to follow the Cox–Merz rule.

肠杆菌sp。在生物柴油行业的甘油副产品上生长的大豆可用于生产增值的胞外多糖（EPS）。在整个培养过程中，根据培养液的形态和流变特性对其培养液进行表征。显微镜观察显示，在培养开始时形成了被EPS包围的细胞聚集体，而最后，聚集体减少了，并观察到了嵌入细胞的EPS基质。培养液的表观粘度随时间增加，这归因于在培养运行的第一阶段中EPS浓度的增加。但是，在最后阶段，复杂培养液中新的聚合物相互作用的产生可能是观察到的粘度增加的原因，因为EPS浓度，平均分子量或化学组成没有明显变化。该肉汤在运行开始时表现出牛顿行为，随着EPS浓度的增加而变为假塑性，并显示遵循Cox–Merz规则。