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Individual Au-Nanocube Based Plasmonic Nanoprobe for Cancer Relevant MicroRNA Biomarker Detection
ACS Sensors ( IF 8.2 ) Pub Date : 2017-09-13 00:00:00 , DOI: 10.1021/acssensors.7b00322 Lei Zhang , Jinghui Wang , Junxia Zhang , Yuqi Liu , Lingzhi Wu , Jingjing Shen , Ying Zhang , Yanling Hu , Quli Fan , Wei Huang 1 , Lianhui Wang
ACS Sensors ( IF 8.2 ) Pub Date : 2017-09-13 00:00:00 , DOI: 10.1021/acssensors.7b00322 Lei Zhang , Jinghui Wang , Junxia Zhang , Yuqi Liu , Lingzhi Wu , Jingjing Shen , Ying Zhang , Yanling Hu , Quli Fan , Wei Huang 1 , Lianhui Wang
Affiliation
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MicroRNA205 (miR-205), as a significant tumor biomarker, is of vital importance for diagnosis of lung cancer and its overexpression patterns have been extensively studied. Here, we report a novel and label-free nanoprobe with high sensitivity and selectivity for miRNA biomarker detection using localized surface plasmon resonance (LSPR) technology on a single DNA modified gold nanocube (AuNC). This method allowed real-time monitoring of the subtle LSPR scattering peak position’s change which was aroused by the variation of dielectric constant in the hybridization process of target miRNA with ssDNA modified on the surface of AuNCs. Notably, the limit of detection of the AuNC-ssDNA probe is up to 5 pM in serum sample, and these results showed that the square structure has more superior sensitivity for design and development of nanoprobe for trace lung cancer relevant miRNAs detection. The better sensing ability and stability of LSPR probe on a AuNC provide potential application to developing a high flux biochip in the future.
中文翻译：
基于单个Au-Nanocube的等离子纳米探针用于癌症相关的MicroRNA生物标志物检测
MicroRNA205（miR-205），作为重要的肿瘤生物标志物，对肺癌的诊断至关重要，其过表达模式已得到广泛研究。在这里，我们报告了一种新颖且无标记的纳米探针，具有高灵敏度和选择性，可在单个DNA修饰的金纳米立方体（AuNC）上使用局部表面等离子体共振（LSPR）技术对miRNA生物标记物进行检测。这种方法可以实时监测微妙的LSPR散射峰位置的变化，这是由靶miRNA与AuNCs表面修饰的ssDNA杂交过程中介电常数变化引起的。值得注意的是，血清样品中AuNC-ssDNA探针的检出限最高为5 pM，这些结果表明，正方形结构对纳米探针的设计和开发具有更高的灵敏度，可用于痕量肺癌相关miRNA的检测。AuNC上的LSPR探针更好的感测能力和稳定性为将来开发高通量生物芯片提供了潜在的应用。
更新日期：2017-09-14
中文翻译：
基于单个Au-Nanocube的等离子纳米探针用于癌症相关的MicroRNA生物标志物检测
MicroRNA205（miR-205），作为重要的肿瘤生物标志物，对肺癌的诊断至关重要，其过表达模式已得到广泛研究。在这里，我们报告了一种新颖且无标记的纳米探针，具有高灵敏度和选择性，可在单个DNA修饰的金纳米立方体（AuNC）上使用局部表面等离子体共振（LSPR）技术对miRNA生物标记物进行检测。这种方法可以实时监测微妙的LSPR散射峰位置的变化，这是由靶miRNA与AuNCs表面修饰的ssDNA杂交过程中介电常数变化引起的。值得注意的是，血清样品中AuNC-ssDNA探针的检出限最高为5 pM，这些结果表明，正方形结构对纳米探针的设计和开发具有更高的灵敏度，可用于痕量肺癌相关miRNA的检测。AuNC上的LSPR探针更好的感测能力和稳定性为将来开发高通量生物芯片提供了潜在的应用。
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