The low abundance of sialo-glycoprotein hampered the separation, enrichment, and analysis of sialo-glycoproteins, which are critical for studying their functions. Here, we designed cleavable amino-oxy functionalized magnetic materials and employed to fast and selective isolate sialo-glycoproteins. This includes the ligation of disulfide-linked amino-oxy-functionalized magnetic nanoparticles with periodate-treated glycoproteins or cells, followed by magnetic separation. A reductive reagent could release the sialo-glycoproteins with small molecular fragments on the terminal of glycan chains, and the sialo-glycoproteins were analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis. On-bead digestion of the peptides were analyzed by tandem mass spectrometry. The results indicated that this method could selectively separate the majority of cell-surface sialo-glycoproteins.

中文翻译：

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二硫键连接的氨基氧基官能化的Fe ₃ O ₄磁性纳米粒子对唾液糖蛋白的选择性裂解提取。

唾液糖蛋白的低丰度阻碍了唾液糖蛋白的分离，富集和分析，这对于研究其功能至关重要。在这里，我们设计了可裂解的氨基氧基官能化磁性材料，并用于快速和选择性分离唾液糖蛋白。这包括将二硫键连接的氨基氧基官能化的磁性纳米粒子与高碘酸盐处理的糖蛋白或细胞连接，然后进行磁分离。还原剂可以释放在聚糖链末端具有小分子片段的唾液糖蛋白，并通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳对唾液糖蛋白进行分析。通过串联质谱分析肽的珠上消化。