A glycan‐stimulated and poly(3,4‐ethylene‐dioxythiophene)s (PEDOT)‐based nanomaterial platform is fabricated to purify circulating tumor cells (CTCs) from blood samples of prostate cancer (PCa) patients. This new platform, phenylboronic acid (PBA)‐grafted PEDOT NanoVelcro, combines the 3D PEDOT nanosubstrate, which greatly enhances CTC capturing efficiency, with a poly(EDOT‐PBA‐co‐EDOT‐EG3) interfacial layer, which not only provides high specificity for CTC capture upon antibody conjugation but also enables competitive binding of sorbitol to gently release the captured cells. CTCs purified by this PEDOT NanoVelcro chip provide well‐preserved RNA transcripts for the analysis of the expression level of several PCa‐specific RNA biomarkers, which may provide clinical insights into the disease.

中文翻译：

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聚糖刺激能够在 PEDOT NanoVelcro 芯片上纯化前列腺癌循环肿瘤细胞，用于 RNA 生物标志物检测


制造了一种聚糖刺激的聚（3,4-乙烯二氧噻吩）（PEDOT）纳米材料平台，用于从前列腺癌（PCa）患者的血液样本中纯化循环肿瘤细胞（CTC）。这个新平台，苯硼酸（PBA）接枝的PEDOT NanoVelcro，结合了3D PEDOT纳米基底，大大提高了CTC捕获效率，与聚（EDOT-PBA- co- EDOT-EG3）界面层相结合，不仅提供了高特异性用于抗体缀合后的 CTC 捕获，还可以实现山梨醇的竞争性结合，以温和地释放捕获的细胞。通过这种 PEDOT NanoVelcro 芯片纯化的 CTC 提供保存完好的 RNA 转录本，用于分析几种 PCa 特异性 RNA 生物标志物的表达水平，这可能提供对该疾病的临床见解。