Relating DNA base-pairing in aqueous media to DNA polymerase fidelity

Nature Reviews Chemistry, Published online: 6 September 2017; doi:10.1038/s41570-017-0074

Controversy surrounds the perceived absence of a relationship between DNA polymerase fidelity (kinetic discrimination) and free energy changes determined from DNA melting studies (thermodynamic discrimination). Thermodynamic discrimination together with aqueous solvent effects can account for kinetic fidelities on the order of those observed experimentally.

中文翻译：

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将水性介质中的DNA碱基配对与DNA聚合酶保真度相关

将水性介质中的DNA碱基配对与DNA聚合酶保真度相关

Nature Reviews Chemistry，在线发布：2017年9月6日；doi：10.1038 / s41570-017-0074

争议围绕着DNA聚合酶保真度（动力学歧视）与根据DNA熔解研究确定的自由能变化（热力学歧视）之间的关系而存在。热力学辨别力与水溶剂效应一起可以解释动力学保真度，其数量级是实验观察到的。