This protocol is an extension to: Nat. Protoc. 5, 503-515 (2010); doi: 10.1038/nprot.2009.235; published online 25 February 2010The FLOTAC is a sensitive, accurate, and precise technique for the diagnosis of protozoan and helminth infections in humans and animals. However, it requires centrifugation, and hence might be out of reach in resource-constrained settings. As an extension of the original FLOTAC protocol, this protocol describes the Mini-FLOTAC technique, a logical evolution of FLOTAC conceived to perform multivalent, qualitative, and quantitative diagnosis of helminth and protozoan infections in human and animal feces, and urine. This has been found to be of most use in the processing of large numbers of samples with rapid laboratory workup, and for veterinary applications directly on-farm. In addition to the Mini-FLOTAC apparatus, we describe the use of the Fill-FLOTAC, a closed system used to facilitate the performance of the first four consecutive steps of the Mini-FLOTAC technique: fecal sample collection and weighing, homogenization, filtration, and filling of the Mini-FLOTAC chambers. Processing of an individual sample using this protocol requires ∼12 min.

中文翻译：

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Mini-FLOTAC技术可用于诊断人类和动物的蠕虫和原生动物感染。

该协议是对以下内容的扩展：Nat。协议。5，503-515（2010）; doi：10.1038 / nprot.2009.235；2010年2月25日在线发布FLOTAC是一种灵敏，准确，精确的技术，用于诊断人和动物的原生动物和蠕虫感染。但是，它需要离心，因此在资源有限的环境中可能无法达到。作为原始FLOTAC协议的扩展，此协议描述了Mini-FLOTAC技术，该技术是FLOTAC的逻辑演进，旨在对人和动物粪便和尿液中的蠕虫和原生动物感染进行多价，定性和定量诊断。已发现这种方法最适用于通过实验室快速处理来处理大量样品，以及直接用于农场的兽医应用。除了Mini-FLOTAC装置外，我们描述了Fill-FLOTAC的使用，这是一个封闭的系统，用于促进Mini-FLOTAC技术的前四个连续步骤的性能：粪便样品的收集和称量，均质化，过滤和Mini-FLOTAC腔室的填充。使用该协议处理单个样品需要大约12分钟。