As member of glycosyl hydrolase family 13, maltooligosaccharide-forming amylases (MFAses) are specific and interesting because of their capacity to hydrolyze starch into functional maltooligosaccharides, which are usually composed of 2–10 α-d-glucopyranosyl units linked by α-1,4 glycosidic linkages. MFAses have been extensively studied during recent decades, and have shown promise in various industrial applications. This review begins by introducing the potential uses of maltooligosaccharides. Then it describes the progress in the identification, assay, action pattern, structure, and modification of MFAses. The review continues with tips concerning the preparation of MFAses, which aim to improve MFAse production to meet the needs of industry. Finally, the industrial uses of MFAses are described, focusing on the production of maltooligosaccharides and application in the bread industry. Recent progress has demonstrated that the MFAses are poised to become important industrial catalysts.

作为糖基水解酶家族13的成员，形成麦芽低聚糖的淀粉酶（MFAses）具有特殊性和趣味性，因为它们具有将淀粉水解为功能性麦芽低聚糖的能力，通常由2-10个α- d组成。-葡萄糖吡喃糖基单元通过α-1,4糖苷键连接。近几十年来，MFA酶已被广泛研究，并已在各种工业应用中显示出希望。这篇综述首先介绍了麦芽低聚糖的潜在用途。然后描述了MFA酶的鉴定，测定，作用模式，结构和修饰的进展。审查继续提供有关MFAse制备的提示，这些提示旨在改善MFAse的生产以满足工业需求。最后，描述了MFA酶的工业用途，重点是麦芽低聚糖的生产及其在面包工业中的应用。最近的进展表明，MFAses有望成为重要的工业催化剂。