Hematopoietic stem cell (HSC) transplantation can restore a new functional hematopoietic system in recipients in cases where the system of the recipient is not functional or for example is leukemic. However, the number of available donor HSCs is often too low for successful transplantation. Expansion of HSCs and thus HSC self-renewal ex vivo would greatly improve transplantation therapy in the clinic. In vivo, HSCs expand significantly in the niche, but establishing protocols that result in HSC expansion ex vivo remains challenging. In this review we discuss current knowledge of niche biology, the intrinsic regulators of HSC self-renewal in vivo, and introduce novel niche-informed strategies of HSC expansion ex vivo.

如果受体的系统无功能或例如发生白血病，则造血干细胞（HSC）移植可在受体中恢复新的功能性造血系统。然而，可用的供体HSC的数量通常太少而无法成功移植。HSC的扩展以及离体HSC自我更新将大大改善临床中的移植治疗。在体内，HSCs在利基市场中显着扩展，但是建立导致HSC体外扩增的方案仍然具有挑战性。在这篇综述中，我们讨论了利基生物学的最新知识，HSC体内自我更新的内在调节剂，并介绍了离体HSC扩增的新颖的利基信息策略。。