CRISPR saturation mutagenesis has the potential to dissect the functional landscape of noncoding regions, but is highly susceptible to false discovery and misinterpretation. As recently published, Canver et al. have now taken the first steps towards addressing these issues by increasing screening resolution and analyzing the effects of off targets on hit calling.

中文翻译：

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CRISPR调控基因组的挑战

CRISPR饱和诱变具有剖析非编码区功能区域的潜力，但极易出现错误发现和曲解。如最近发表的，Canver等。现在已经采取了第一步措施，通过提高筛选分辨率并分析偏离目标对点击率的影响来解决这些问题。