A culture-based survey of staining fungi on oil-treated timber after outdoor exposure in Australia and the Netherlands uncovered new taxa in Pezizomycotina. Their taxonomic novelty was confirmed by phylogenetic analyses of multi-locus sequences (ITS, nrSSU, nrLSU, mitSSU, RPB1, RPB2, and EF-1α) using multiple reference data sets. These previously unknown taxa are recognised as part of a new order (Superstratomycetales) potentially closely related to Trypetheliales (Dothideomycetes), and as a new species of Cyanodermella, C. oleoligni in Stictidaceae (Ostropales) part of the mostly lichenised class Lecanoromycetes. Within Superstratomycetales a single genus named Superstratomyces with three putative species: S. flavomucosus, S. atroviridis, and S. albomucosus are formally described. Monophyly of each circumscribed Superstratomyces species was highly supported and the intraspecific genetic variation was substantially lower than interspecific differences detected among species based on the ITS, nrLSU, and EF-1α loci. Ribosomal loci for all members of Superstratomyces were noticeably different from all fungal sequences available in GenBank. All strains from this genus grow slowly in culture, have darkly pigmented mycelia and produce pycnidia. The strains of C. oleoligni form green colonies with slimy masses and develop green pycnidia on oatmeal agar. These new taxa could not be classified reliably at the class and lower taxonomic ranks by sequencing from the substrate directly or based solely on culture-dependent morphological investigations. Coupling phenotypic observations with multi-locus sequencing of fungi isolated in culture enabled these taxonomic discoveries. Outdoor situated timber provides a great potential for culturable undescribed fungal taxa, including higher rank lineages as revealed by this study, and therefore, should be further explored.

对澳大利亚和荷兰户外暴露后的油处理木材上的染色真菌进行的一项基于培养的调查发现了盘菌亚门的新类群。使用多个参考数据集对多位点序列（ITS、nrSSU、nrLSU、mitSSU、 RPB1 、 RPB2和EF-1α ）进行系统发育分析，证实了它们的分类学新颖性。这些以前未知的类群被认为是可能与Trypetheliales ( Dothideomycetes ) 密切相关的新目 ( Superstratomycetales ) 的一部分，并且被认为是Stictidaceae ( Ostropales ) 中蓝皮菌 ( Cyanodermella ) 的新种， C. oleoligni，属于大多数地衣化的Lecanoromycetes的一部分。在Superstratomycetales中，正式描述了一个名为Superstratomyces的单一属，该属具有三个假定的物种： S. flavomucosus 、 S. atroviridis和S. albomucosus 。每个限制性Superstratomyces物种的单系性得到了高度支持，并且种内遗传变异大大低于基于 ITS、nrLSU 和EF-1α位点检测到的物种间差异。 Superstratomyces所有成员的核糖体位点与 GenBank 中可用的所有真菌序列明显不同。该属的所有菌株在培养物中生长缓慢，具有深色菌丝体并产生分生孢子器。 C. oleoligni菌株形成具有粘液团块的绿色菌落，并在燕麦琼脂上形成绿色分生孢子器。 通过直接从底物测序或仅基于依赖于培养物的形态学研究，无法对这些新分类群在类和较低分类等级上进行可靠分类。将表型观察与培养物中分离的真菌的多位点测序相结合，实现了这些分类学发现。室外木材为可培养的未描述的真菌类群提供了巨大的潜力，包括本研究揭示的更高等级的谱系，因此应该进一步探索。