Direct, Label-Free, and Rapid Transistor-Based Immunodetection in Whole Serum,ACS Sensors

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Direct, Label-Free, and Rapid Transistor-Based Immunodetection in Whole Serum
ACS Sensors ( IF 8.2 ) Pub Date : 2017-08-30 00:00:00 , DOI: 10.1021/acssensors.7b00187
Óscar Gutiérrez-Sanz ₁ , Nesha M. Andoy ₁ , Marcin S. Filipiak _{1,

2} , Natalie Haustein _{1,

3} , Alexey Tarasov _{1,

2}

Affiliation

Transistor-based biosensors fulfill many requirements posed upon transducers for future point-of-care diagnostic devices such as scalable fabrication and label-free and real-time quantification of chemical and biological species with high sensitivity. However, the short Debye screening length in physiological samples (<1 nm) has been a major drawback so far, preventing direct measurements in serum. In this work, we demonstrate how tailoring the sensing surface with short specific biological receptors and a polymer polyethylene glycol (PEG) can strongly enhance the sensor response. In addition, the sensor performance can be dramatically improved if the measurements are performed at elevated temperatures (37 °C instead of 21 °C). With this novel approach, highly sensitive and selective detection of a representative immunosensing parameter—human thyroid-stimulating hormone—is shown over a wide measuring range with subpicomolar detection limits in whole serum. To the best of our knowledge, this is the first demonstration of direct immunodetection in whole serum using transistor-based biosensors, without the need for sample pretreatment, labeling, or washing steps. The presented sensor is low-cost, can be easily integrated into portable diagnostics devices, and offers a competitive performance compared to state-of-the-art central laboratory analyzers.

中文翻译：

直接，无标签，基于晶体管的全血清快速免疫检测

基于晶体管的生物传感器可以满足传感器对未来医疗现场诊断设备提出的许多要求，例如可扩展的制造以及对化学和生物物种进行高灵敏度的无标记和实时定量分析。但是，到目前为止，生理样品中短的德拜（Debye）筛选长度（<1 nm）一直是主要缺点，无法在血清中进行直接测量。在这项工作中，我们演示了如何使用短的特定生物受体和聚合物聚乙二醇（PEG）剪裁感测表面如何极大地增强传感器响应。此外，如果在升高的温度（37°C而不是21°C）下进行测量，则传感器性能将得到显着改善。有了这种新颖的方法，可以在很宽的测量范围内显示出具有代表性的免疫传感参数（人类甲状腺刺激激素）的高灵敏度和选择性检测，整个血清的检测极限为亚皮摩尔。据我们所知，这是使用基于晶体管的生物传感器在全血清中进行直接免疫检测的首次演示，而无需进行样品预处理，标记或洗涤步骤。与最先进的中央实验室分析仪相比，该传感器成本低廉，可以轻松集成到便携式诊断设备中，并具有竞争优势。无需样品预处理，标记或清洗步骤。与最先进的中央实验室分析仪相比，该传感器成本低廉，可以轻松集成到便携式诊断设备中，并具有竞争优势。无需样品预处理，标记或清洗步骤。与最先进的中央实验室分析仪相比，该传感器成本低廉，可以轻松集成到便携式诊断设备中，并具有竞争优势。

更新日期：2017-08-30

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